Eosinophil peroxidase over-expression predicts the clinical outcome of patients with primary lung adenocarcinoma
Eosinophil peroxidase over-expression predicts the clinical outcome of patients with primary lung adenocarcinoma
复制标题
嗜酸性粒细胞过氧化物酶过度表达可预测原发性肺腺癌患者的临床结果
DOI：
10.7150/jca.24314
复制
发表时间：
2019-01
影响因子：
3.9
通讯作者：
Fang Zhang
中科院分区：
文献类型：
--
作者：
liang ye;Hongying Wang;Huijuan Li;Hongbing Liu;Tangfeng Lv;Yong Song;Fang Zhang
嗜酸性粒细胞过氧化物酶（EPO）是一种在嗜酸性粒细胞中大量表达的血红素蛋白，参与催化细胞毒性氧化剂，与癌症、哮喘和过敏性炎症性疾病的发病机制相关。迄今为止，其在肺癌发病机制中的作用仍不清楚。采用Real-time PCR和Western blotting方法分别检测肺腺癌组织和癌旁正常肺组织中EPO的表达。此外，EPO蛋白表达在90个肺腺癌（AD）样本证实与免疫组织化学（IHC）使用组织芯片。同时，探讨EPO与肺腺癌患者临床病理特征及疾病预后的关系，结果显示，EPO在肺腺癌组织中的mRNA和蛋白表达均显著高于癌旁正常肺组织（P<0.05）。EPO过表达与肿瘤病理分期（p-TNM分期，P=0.017）和淋巴结转移（P=0.027）显著相关。根据Kaplan-Meier生存曲线，EPO过表达患者的生存时间短于EPO水平低的患者（P=0.017）。采用多因素考克斯回归模型分析影响预后的因素，结果表明N分期对预后有显著影响，（HR=0.965，95% CI=0.328-1.359，P=0.008），p-TNM分期EPO蛋白高表达（HR = 3.145，95% CI = 2.016 - 5.519，P =0.018）是肺AD预后的独立影响因素。总之，EPO表达增加可作为肺AD患者预后不良的生物标志物。
Eosinophil peroxidase (EPO), a heme protein abundantly expressed in eosinophils, involves in the catalysis of cytotoxic oxidants associated with the pathogenesis of cancer, asthma, and allergic inflammatory disorders. To date, its roles in the pathogenesis of lung cancer are still not known. We determined the expression of EPO in the lung adenocarcinoma tissues and the normal adjacent lung tissues using Real-time PCR and Western blotting analysis, respectively. Also, EPO protein expression in 90 lung adenocarcinoma (AD) samples were confirmed with immunohistochemistry (IHC) using tissue microarrays. Meanwhile, we investigated the association between EPO and the clinicopathological characteristics and disease prognosis in the pulmonary adenocarcinoma patients, which demonstrated that EPO mRNA and protein were significantly higher in lung AD tissues that those of the adjacent normal lung tissues (P<0.05). EPO overexpression was significantly correlated with pathologic-tumour nodes metastasen stage (p-TNM stage, P=0.017) and lymph node metastasis (P=0.027). Patients with EPO overexpression showed shorter survival time than those with low EPO levels (P=0.017), according to the Kaplan-Meier survival curve. Furthermore, a multivariate Cox regression model was utilized to analyze the prognostic factors, which indicated that N stage (HR=0.965, 95% CI=0.328-1.359, P=0.008), p-TNM Stage (HR=3.127, 95% CI =2.463-5.015, P=0.021) and high EPO protein expression (HR=3.145, 95% CI=2.016-5.519, P=0.018) were independent factors for the prognosis of lung AD. In conclusion, increased EPO expression could be used as a biomarker for lung AD patients with poor prognosis.
登录
查看更多内容
影响因子：
7.4
作者：
Reuter, Simone;Gupta, Subash C.;Chaturvedi, Madan M.;Aggarwal, Bharat B.
通讯作者：
Aggarwal, Bharat B.
DOI：
10.1891/9780826121646.0002
发表时间：
2018-09
期刊：
Cancer Rehabilitation
影响因子：
--
作者：
K. Miller;R. Siegel;R. Khan;A. Jemal
通讯作者：
K. Miller;R. Siegel;R. Khan;A. Jemal
DOI：
10.1016/j.biocel.2015.09.006
发表时间：
2015-11
期刊：
The international journal of biochemistry & cell biology
影响因子：
--
作者：
V. Panagopoulos;Irene Zinonos;D. Leach;S. Hay;Vasilios Liapis;A. Zysk;W. Ingman;M. DeNichilo;A. Evdokiou
通讯作者：
V. Panagopoulos;Irene Zinonos;D. Leach;S. Hay;Vasilios Liapis;A. Zysk;W. Ingman;M. DeNichilo;A. Evdokiou
影响因子：
6.4
作者：
Lehtonen, ST;Svensk, AM;Kinnula, VL
通讯作者：
Kinnula, VL
DOI：
10.1016/s0098-1672(09)79086-1
发表时间：
2010
期刊：
Yearbook of Diagnostic Radiology
影响因子：
--
作者：
M. R. D. Christenson
通讯作者：
M. R. D. Christenson