Cluster organization of the genes of Streptomyces pristinaespiralis involved in pristinamycin biosynthesis and resistance elucidated by pulsed-field gel electrophoresis
Cluster organization of the genes of Streptomyces pristinaespiralis involved in pristinamycin biosynthesis and resistance elucidated by pulsed-field gel electrophoresis
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DOI：
10.1046/j.1365-2672.1999.00955.x
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发表时间：
1999-12-01
影响因子：
4
通讯作者：
Crouzet, J
中科院分区：
文献类型：
--
作者：
Bamas-Jacques, N;Lorenzon, S;Crouzet, J
普氏链霉菌合成普里斯霉素，它是链球菌抗生素家族的一员，它由两种化学上不相关的化合物组成，称为普里斯霉素I和普里斯霉素II。为了估计步骤的大小。为了阐明已鉴定的原始霉素生物合成基因和抗性基因的组织结构，决定使用脉冲场凝胶电泳技术。结果表明，链霉菌属(Strep.)原始链霉菌的染色体是线形的，大小约7580kb，如先前对几种链霉菌所显示的那样。杂交结果表明，除多药耐药基因ptr外，原生霉素I和原生霉素II的生物合成基因和抗性基因呈点状分布，呈单一的大簇分布，覆盖不到200kb，占染色体总大小的2.6%。讨论了这种遗传组织的后果和意义。
Streptomyces pristinaespiralis synthesizes pristinamycin, a member of the streptogramin antibiotic family which consists of a mixture of two types of chemically unrelated compounds named pristinamycins I and pristinamycins II. In order to estimate the size of the Step. pristinaespiralis chromosome and to elucidate the organization of the pristinamycin biosynthetic and resistance genes already identified, it was decided to use the pulsed-field gel electrophoresis technique. Results indicate that the Strep. pristinaespiralis chromosome Is linear and about 7580 kb, as previously shown for several or-her Streptomyces species. By hybridization, it could be shown that the biosynthetic and resistance genes for pristinamycins I and pristinamycins II, except for the multidrug resistance gene ptr, are interspersed and seem to be organized as a single large cluster, covering less than 200 kb corresponding to 2.6% of the total size of the chromosome. The consequences and significance of such a genetic organization are discussed.