Novel subtyping of intestinal metaplasia in the human stomach - Brain-type glycogen phosphorylase expression in the proliferative zone and its relationship with carcinogenesis
Novel subtyping of intestinal metaplasia in the human stomach - Brain-type glycogen phosphorylase expression in the proliferative zone and its relationship with carcinogenesis
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DOI：
10.1093/ajcp/109.2.181
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发表时间：
1998-02-01
影响因子：
3.5
通讯作者：
Ogawa, M
中科院分区：
文献类型：
--
作者：
Matsuzaki, H;Shimada, S;Ogawa, M
尽管有报道表明不完全型肠上皮化生（IM）与癌密切相关；大量数据显示特定类型的IM与肠型癌之间没有明显的关系。本研究的目的是从致癌的角度使用脑型糖原磷酸化酶 (BGP) 建立一种新的 IM 分类。通过聚合酶链式反应分析，胃癌中唯一表达的亚型是 BGP。我们使用特异性抗 BGP 抗体研究了 136 例胃癌和邻近 IM 标本，及其与 IM 亚型、增殖细胞核抗原标记指数和各种癌基因产物的相关性。脑型糖原磷酸化酶在80.5%的肠型癌和18.8%的弥漫型癌中表达，在87.5%和41.6%的邻近癌灶的IM生成区中表达，而在正常胃粘膜中没有观察到反应性。肠型癌中癌和IM的阳性比例显着高于弥漫型。 IM生殖细胞中BGP的表达与常规型IM无明显相关性。表达BGP的肠化生在增殖状态下明显高于未表达BGP的肠化生，并且其中一些共表达的肠化生在生殖细胞中积累了p53。 IM与生殖细胞中的BGP与肠型癌的关系明显比常规IM亚型与胃癌的关系更密切。肠型癌可能是由一些具有 BGP 的增殖细胞引起的。
Although reports have suggested the incomplete type of intestinal metaplasia (IM) had a close correlation with carcinoma; considerable data showed no apparent relationship between the particular type of IM and the intestinal type carcinoma. The purpose of this study was to establish a novel classification of IM using brain-type glycogen phosphorylase (BGP) from a carcinogenetic viewpoint. The only isoform expressed in gastric cancer was BGP using polymerase chain reaction analysis. We studied 136 specimens with gastric carcinoma and the adjacent IM using specific anti-BGP antibody with its correlation to subtypes of IM, proliferating cell nuclear antigen-labeling index, and various oncogene products. Brain-type glycogen phosphorylase was expressed in 80.5% of the intestinal type and 18.8% of the diffuse type of carcinoma and in 87.5% and 41.6% in the generative zone of IM adjacent to cancer foci, respectively, whereas no reactivity was observed in the normal gastric mucosa. The proportion of the positivity in the cancer and IM was significantly greater in the intestinal-type carcinoma than in the diffuse type. The expression of BGP in the generative cells of IM had no significant correlation with the conventional type of IM. Intestinal metaplasias with BGP expression were significantly higher in a proliferating state than in those without BGP, and some of them that were coexpressed accumulated p53 in the generative cells. The relationship between IM with BGP in the generative cells and intestinal-type carcinoma was apparently closer than the conventional subtype of IM and gastric cancer. Intestinal-type carcinoma might arise from some of these proliferating cells with BGP.