The RNA Polymerase II core subunit RPB-9 directs transcriptional elongation at piRNA loci in Caenorhabditis elegans
The RNA Polymerase II core subunit RPB-9 directs transcriptional elongation at piRNA loci in Caenorhabditis elegans
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DOI：
10.1101/2020.05.01.070433
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发表时间：
2020-05
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通讯作者：
Ahmet C. Berkyurek;G. Furlan;Lisa Lampersberger;Toni Beltran;Eva-Maria Weick;Emily Nischwitz;I. C. Navarro;Fabian Braukmann;Alper Akay;Jonathan Price;F. Butter;P. Sarkies;E. Miska
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作者：
Ahmet C. Berkyurek;G. Furlan;Lisa Lampersberger;Toni Beltran;Eva-Maria Weick;Emily Nischwitz;I. C. Navarro;Fabian Braukmann;Alper Akay;Jonathan Price;F. Butter;P. Sarkies;E. Miska
Piwi相互作用RNAs(PiRNAs)是基因组编码的小RNA，在许多动物中调节生殖细胞的发育和维持生殖系的完整性。成熟的piRNA利用Piwi ArgAerte蛋白来沉默互补转录产物，包括转座元件和内源基因。PiRNA的生物发生机制多种多样，目前仍知之甚少。在这里，我们确定了线虫线虫中piRNA介导的沉默所必需的RNA聚合酶II(RNA PolII)核心亚单位RPB-9。RPB-9突变体未能启动可遗传的piRNA介导的基因沉默。此外，我们发现RPB-9是抑制线虫种系中的两个DNA转座子家族和一个体细胞基因子集所必需的。我们提供了遗传和生化证据，证明RPB-9是piRNA生物发生所必需的。我们证明RPB-9在内源piRNA基因座上促进转录延长/终止。我们得出结论，作为其快速进化的一部分，piRNA途径已经采用了另一种古老的机制，这一次是为了高保真转录。
PIWI-interacting RNAs (piRNAs) are genome-encoded small RNAs that regulate germ cell development and maintain germline integrity in many animals. Mature piRNAs engage Piwi Argonaute proteins to silence complementary transcripts, including transposable elements and endogenous genes. piRNA biogenesis mechanisms are diverse and remain poorly understood. Here, we identify the RNA Polymerase II (RNA Pol II) core subunit RPB-9 as required for piRNA-mediated silencing in the nematode Caenorhabditis elegans. rpb-9 mutants fail to initiate heritable piRNA-mediated gene silencing. Furthermore, we show that RPB-9 is required to repress two DNA transposon families and a subset of somatic genes in the C. elegans germline. We provide genetic and biochemical evidence that RPB-9 is required for piRNA biogenesis. We demonstrate that RPB-9 acts to promote transcriptional elongation/termination at endogenous piRNA loci. We conclude that as a part of its rapid evolution the piRNA pathway has co-opted another ancient machinery, this time for high-fidelity transcription.