Stromal development in the ventral prostate, anterior prostate and seminal vesicle of the rat.
Stromal development in the ventral prostate, anterior prostate and seminal vesicle of the rat.
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DOI：
10.1159/000147794
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发表时间：
1996
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影响因子：
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通讯作者：
Simon W. Hayward;Laurence S. Baskin;Peter C. Haughney;A. Cunha;B. A. Foster;Rajvir Dahiya;G. Prins;Cunha Gr
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文献类型：
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作者：
Simon W. Hayward;Laurence S. Baskin;Peter C. Haughney;A. Cunha;B. A. Foster;Rajvir Dahiya;G. Prins;Cunha Gr
前列腺和精囊（SV）是男性生殖道的雄激素依赖性分泌腺。它们产生大量的精液分泌物。本研究的目的是检查和文件的个体发育的基质成熟的大鼠前列腺前腹侧和SV。这些器官在胎儿发育期间具有松散组织的细胞间充质。在前列腺发育过程中，间充质浓缩形成平滑肌鞘，直接包围上皮细胞，单个导管之间有疏松的结缔组织。在SV中，称为固有层的疏松结缔组织层位于上皮和发育中的肌肉之间。平滑肌α-肌动蛋白，肌球蛋白，结蛋白，层粘连蛋白，黏着斑蛋白，波形蛋白和雄激素受体（AR）的表达进行了检查，通过免疫细胞化学方法在出生前和出生后的发展时期。第一个被检测到的标志物是波形蛋白，它最初在整个间充质中被发现。在发育过程中，波形蛋白主要局限于前列腺的导管间组织和SV的固有层。平滑肌标志物以有序的顺序表达，从近端到远端的方式沿着前列腺导管，从尿道到尖端。α-肌动蛋白的表达之后是黏着斑蛋白、肌球蛋白、结蛋白和层粘连蛋白。这些标记物定位于发育中的平滑肌鞘，并且在前列腺的导管间组织或SV的固有层中不表达。器官培养实验表明，雄激素是平滑肌鞘分化所必需的。成年大鼠去势表明，雄激素是维持平滑肌分化所必需的。在去势动物中，间质相对较厚，但密度低于完整动物。去势后，平滑肌标志物的表达顺序丧失，其表达在发育过程中的相反顺序。在长期去势中，检测到α-肌动蛋白、波形蛋白和少量黏着斑蛋白。妊娠16天，在尿生殖窦上皮周围的间充质中首次检测到AR。随着发育的进展，AR的表达变得更加广泛，出生后在整个间充质中发现。随着平滑肌的成熟，AR的基质表达定位于上皮周围的肌鞘。在前列腺中，导管间结缔组织显示非常低水平的AR表达。在SV中，在固有层中也观察到AR。总之，大鼠前列腺和SV的间质分化和去分化被认为是雄激素依赖的过程，具有特定标志物的有序的个体发育表达。
The prostate and seminal vesicle (SV) are androgen-dependent secretory glands of the male genital tract. They produce the bulk of the seminal secretions. The object of the present study was to examine and document the ontogeny of stromal maturation in the rat anterior and ventral prostate and SV. These organs have a loosely organized cellular mesenchyme during fetal development. During prostatic development the mesenchyme condensed to form smooth muscle sheaths immediately surrounding the epithelium, with looser connective tissue between individual ducts. In the SV, a loose connective tissue layer called the lamina propria lies between the epithelium and developing muscle. Smooth muscle alpha-actin, myosin, desmin, laminin, vinculin, vimentin and androgen receptor (AR) expression were examined by immunocytochemical methods during the pre- and postnatal developmental periods. The first marker to be detected was vimentin, which was initially found throughout the mesenchyme. During development vimentin became mostly restricted to the interductal tissue of the prostate and the lamina propria of the SV. Smooth muscle markers were expressed in an orderly sequence in a proximal to distal manner along prostatic ducts, from the urethra towards the tips. Expression of alpha-actin was followed by vinculin, myosin, desmin, and laminin. These markers became localized to the developing smooth muscle sheaths and were not expressed in the interductal tissue of the prostate or the lamina propria of the SV. Organ culture experiments demonstrated that androgens were required for the differentiation of smooth muscle sheaths. Castration of adult rats demonstrated that androgens were required to maintain smooth muscle differentiation. In castrates, the stroma was relatively thicker but less dense than in intact animals. Following castration, expression of the smooth muscle markers was lost sequentially in the reverse order of their expression during development. In long-term castrates alpha-actin, vimentin and a small amount of vinculin were detected. AR were first detected in the urogenital sinus mesenchyme immediately surrounding the epithelium at 16 days of gestation. As development progressed expression of AR became more widespread, and postnatally was found throughout the mesenchyme. As maturation of smooth muscle occurred, stromal expression of AR became localized to the muscular sheath immediately surrounding the epithelium. In the prostate the interductal connective tissue displayed very low levels of AR expression. In the SV, AR were also observed in the lamina propria. In summary, stromal differentiation and dedifferentiation in the rat prostate and SV were found to be androgen-dependent processes with ordered sequential ontogenic expression of specific markers.