Heritable improvement of frost tolerance in winter wheat by in vitro selection of hydroxyproline-resistant proline overproducing mutants
Heritable improvement of frost tolerance in winter wheat by in vitro selection of hydroxyproline-resistant proline overproducing mutants
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DOI：
10.1023/a:1002946622376
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发表时间：
1997-01-01
期刊：
影响因子：
1.9
通讯作者：
Jurgens, HU
中科院分区：
文献类型：
--
作者：
Dorffling, K;Dorffling, H;Jurgens, HU
在先前的研究（Dorffling等人，142，222-225，1993）-冬小麦（Triticum aestivum cv.乔3063）的抗霜性和脯氨酸含量的遗传力进行了研究。与野生型植株和离体对照相比，用野生型植株花粉授粉的F1代植株具有更高的平均抗冻性（较低的LT（50）值）和更高的脯氨酸水平。在自花授粉获得的F-2代中，观察到Hyp-lines在耐霜冻性和脯氨酸含量方面以3：1的表型比例分离。随后在F-2中选择极端，在F-3代中产生纯合植物。低LT（50）值和增加脯氨酸含量在这些F-3植物显着相关。此外，在F_4代整株研究中，也观察到其中一个突变体的抗冻性提高，这一结果与冬大麦的平行研究结果相似，为通过体外选择方法获得的“抗冻性提高”和“脯氨酸含量增加”性状的遗传力提供了有力的证据。这种突变似乎是由一个不完全显性基因引起的。F3代Hyp-proliferation的耐霜性值与7个耐霜性不同的标准品种的耐霜性值的比较表明，通过所述的体外选择方法，耐霜性有相当大的提高。
In a previous study (Dorffling et al., J. Plant Physiol. 142, 222-225, 1993) in vitro-selection and regeneration of hydroxyproline (Hyp)-resistant lines of winter wheat (Triticum aestivum cv. Jo 3063) with increased frost tolerance and increased proline contents was reported.In this study the heritability of these traits was investigated. The F-1 progenies derived from the pollination of regenerated plants with pollen from wild type plants developed higher mean frost tolerance (lower LT(50) values) and higher proline levels compared with in vitro-controls and wild type plants. In the F-2 generation, which was obtained by self-pollination, segregation of the Hyp-lines in a 3:1 phenotypic ratio with regard to the traits frost tolerance and proline content was observed. Subsequent selection of extremes in the F-2 yielded homozygous plants in the F-3 generation. Lower LT(50) values and increased proline contents in those F-3 plants were significantly correlated. Furthermore, improved frost tolerance of one of the selected mutants was also observed in studies with whole plants in the F-4 generation.These results, which are similar to results of parallel studies on winter barley, provide strong evidence for the heritability of the traits 'increased frost tolerance' and 'increased proline content' obtained by this in vitro-selection procedure. The mutation seems to be due to a single incompletely dominant gene. A comparison of frost tolerance values from F-3 Hyp-progenies with those of seven standard varieties differing in frost tolerance indicates a considerable improvement of frost tolerance by the described in vitro-selection method.