Inheritance Characteristics of Microsatellite DNA Loci in Experimental Families of Japanese Flounder Paralichthys olivaceus
Inheritance Characteristics of Microsatellite DNA Loci in Experimental Families of Japanese Flounder Paralichthys olivaceus
复制标题
牙鲆实验家系微卫星DNA位点的遗传特征
DOI：
10.1007/s10126-001-0052-z
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发表时间：
2001
影响因子：
3
通讯作者：
M. Hara
中科院分区：
文献类型：
--
作者：
M. Sekino;M. Hara
日本牙鲆 5 个实验科的微卫星 DNA 基因座的等位基因分离模式以及天然牙鲆中与 Hardy-Weinberg 期望 (HWE) 偏差的基因型频率。研究了橄榄种群以评估遗传性。在检查的 12 个微卫星基因座中，1 个基因座可能因特定等位基因的不可重复聚合酶链反应扩增而导致杂合个体评分错误。在其余 11 个位点上，几乎所有等位基因均按照孟德尔传播进行分离，并且在自然群体中观察到的基因型频率与 HWE 一致。这里展示的结果将提供有用的信息，支持这些微卫星基因座作为可遗传基因座的适用性。橄榄遗传标记。
Allele segregating patterns of microsatellite DNA loci in 5 experimental families of Japanese flounderParalichthys olivaceusand genotype frequencies for deviation from Hardy-Weinberg expectations (HWE) in naturalP. olivaceuspopulations were studied to assess inheritability. Of the 12 microsatellite loci examined, 1 locus had a possibility of scoring errors of heterozygous individuals caused by unreproducible polymerase chain reaction amplifications of a particular allele. At the remaining 11 loci, almost all of alleles were segregated according to Mendelian transmission, and observed genotype frequencies in natural populations were consistent with HWE. The results demonstrated here would provide useful information supporting the suitability of these microsatellite loci as inheritableP. olivaceusgenetic markers.