ANALYSIS OF POTATO-VIRUS-X COAT PROTEIN GENES IN RELATION TO RESISTANCE CONFERRED BY THE GENES NX, NB AND RX1 OF POTATO
ANALYSIS OF POTATO-VIRUS-X COAT PROTEIN GENES IN RELATION TO RESISTANCE CONFERRED BY THE GENES NX, NB AND RX1 OF POTATO
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DOI：
10.1099/0022-1317-76-8-2057
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发表时间：
1995-08-01
影响因子：
3.8
通讯作者：
BAULCOMBE, D
中科院分区：
文献类型：
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作者：
CRUZ, SS;BAULCOMBE, D
确定了八种先前未表征的马铃薯 X 病毒 (PVX) 株的外壳蛋白基因核苷酸序列。推导的氨基酸序列的比较表明，可以根据蛋白质长度和总体氨基酸同一性区分两类PVX外壳蛋白，称为X型和B型。总共有 14 个氨基酸位置，所有 X 型蛋白都与所有 B 型蛋白不同。 PVX 外壳蛋白是病毒与携带 Nx 或 Rx1 抗性基因的马铃薯之间相互作用结果的主要病毒决定因素。测试了不同的 PVX 品系克服三个马铃薯抗性基因 Nx、Nb 和 Rx1 赋予的抗性的能力。发现所有对携带 Nx 抗性基因的马铃薯品种无毒力的菌株都具有 X 型外壳蛋白，而能够克服 Nx 抗性的菌株则具有 B 型外壳蛋白。
The coat protein gene nucleotide sequences from eight previously uncharacterized strains of potato virus X (PVX) were determined. Comparison of the deduced amino acid sequences showed that two classes of PVX coat protein, designated types X and B, could be distinguished based on protein length and overall amino acid identities. In all there were 14 amino acid positions where all of the type X proteins differed from all of the type B proteins. The PVX coat protein is the principal viral determinant of the outcome of interactions between the virus and potatoes carrying either the Nx or Rx1 resistance genes. The different strains of PVX were tested for their ability to overcome resistance conferred by three potato resistance genes: Nx, Nb and Rx1. All of the strains that were avirulent on potato cultivars carrying the Nx resistance gene were found to have type X coat proteins whereas strains capable of overcoming the Nx resistance had type B coat proteins.