Contribution of zona proteins to oocyte growth.
Contribution of zona proteins to oocyte growth.
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发表时间：
2007
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通讯作者：
A. Hasegawa;K. Koyama
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文献类型：
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作者：
A. Hasegawa;K. Koyama
透明带是一种细胞外基质，包围着发育中的卵母细胞、排卵卵和植入前胚胎。在小鼠体内，透明带由三种糖蛋白组成，分别为ZPA、ZPB和ZPC。ZPA和ZPC基因敲除小鼠由于卵泡发育不完全而不育。本文综述了ZPA在卵母细胞生长和存活中的作用，以及ZPA被整合到卵带结构中的机制。在卵母细胞颗粒细胞复合体(OGC)中注射小鼠ZPA的短干扰RNA(SiRNAs)，在α-MEM中培养5d。这些卵母细胞的存活率和存活卵母细胞的直径明显低于对照卵母细胞。透明带形态也异常。这些观察表明，小鼠ZPA蛋白有助于卵母细胞的生长和透明带的形成。此外，携带猪ZPA的微型基因构建体被转移到小鼠体内，以检测该基因是否表达，并将蛋白产物整合到小鼠的透明带中。猪ZPA是由小鼠卵母细胞合成和分泌的，但不用于组装嵌合的透明带。这些结果表明，猪和小鼠对透明带组装有不同的要求。
The zona pellucida is an extracellular matrix surrounding growing oocytes, ovulated eggs and preimplantation embryos. In mice, the zona pellucida is composed of three glycoproteins designated as ZPA, ZPB and ZPC. ZpA and ZpC knockout mice are infertile due to incomplete folliculogenesis. In this article, the role of ZPA in the growth and survival of oocytes and the mechanism by which it is incorporated into zona structure is described. Oocyte-granulosa cell complexes (OGCs) were injected with short interfering RNAs (siRNAs) of mouse ZpA and cultured in alpha-MEM for 5 days. The survival rate of these oocytes and the diameter of surviving oocytes were significantly lower as compared to the control oocytes. The zona pellucida was also abnormal in morphology. These observations suggest that mouse ZPA protein contributes to oocyte growth as well as zona pellucida formation. In addition, a minigene construct carrying pig ZpA was transferred into mice to examine whether the gene is expressed and the protein product incorporated into the mouse zona pellucida. Pig ZPA was synthesized and secreted by the mouse oocytes but not used to assemble a chimeric zona pellucida. These results suggest that there are different requirements for zona assembly in pigs and mice.