Development of an effective protein-labeling system based on smart fluorogenic probes
Development of an effective protein-labeling system based on smart fluorogenic probes
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DOI：
10.1007/s00775-019-01669-y
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发表时间：
2019-05
期刊：
影响因子：
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通讯作者：
S. I. Reja;Masafumi Minoshima;Y. Hori;K. Kikuchi
中科院分区：
文献类型：
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作者：
S. I. Reja;Masafumi Minoshima;Y. Hori;K. Kikuchi
蛋白质是生命系统的重要组成部分，在各种生理功能中起着至关重要的作用。蛋白质的荧光成像是监测蛋白质动力学的有力工具。基于荧光蛋白（FP）的标记方法经常用于监测细胞蛋白质的运动和相互作用。然而，也已经开发了允许使用合成荧光探针来靶向感兴趣的蛋白质（POI）的替代方法。合成的荧光探针与基于FP的标记方法相比具有各种优点。它们的尺寸比荧光蛋白小，提供各种颜色，并具有改进的光化学特性。有各种基于化学发光的标记技术可用于用合成探针标记POI。本文综述了近年来蛋白质标记系统的发展，特别是SNAP标记、BL标记和PYP标记系统，并对这些系统中荧光标记的靶蛋白的荧光行为进行了研究。我们也讨论了这些蛋白质标记系统的智能荧光探针及其应用。荧光蛋白质标记将是未来细胞生物学研究中研究复杂生物现象的有用工具。图形摘要
AbstractProteins are an important component of living systems and play a crucial role in various physiological functions. Fluorescence imaging of proteins is a powerful tool for monitoring protein dynamics. Fluorescent protein (FP)-based labeling methods are frequently used to monitor the movement and interaction of cellular proteins. However, alternative methods have also been developed that allow the use of synthetic fluorescent probes to target a protein of interest (POI). Synthetic fluorescent probes have various advantages over FP-based labeling methods. They are smaller in size than the fluorescent proteins, offer a wide variety of colors and have improved photochemical properties. There are various chemical recognition-based labeling techniques that can be used for labeling a POI with a synthetic probe. In this review, we focus on the development of protein-labeling systems, particularly the SNAP-tag, BL-tag, and PYP-tag systems, and understanding the fluorescence behavior of the fluorescently labeled target protein in these systems. We also discuss the smart fluorogenic probes for these protein-labeling systems and their applications. The fluorogenic protein labeling will be a useful tool to investigate complex biological phenomena in future work on cell biology.Graphical abstract