Bioconversion of Sodium Dodecyl Sulphate to Rhamnolipid by Pseudomonas aeruginosa: A Novel and Cost-Effective Production Strategy
Bioconversion of Sodium Dodecyl Sulphate to Rhamnolipid by Pseudomonas aeruginosa: A Novel and Cost-Effective Production Strategy
复制标题
DOI：
10.1007/s12010-012-9988-x
复制
发表时间：
2013-01-01
影响因子：
3
通讯作者：
Jisha, M. S.
中科院分区：
文献类型：
--
作者：
Rebello, Sharrel;Asok, Aju K.;Jisha, M. S.
由于其经济生产的高度限制，目前鼠李糖脂在工业中的应用受到限制。在这种情况下，十二烷基硫酸钠（SDS）作为碳源的新用途可以作为有前途的成本效益策略。通过筛选有效的 SDS 生物降解剂，分离出能够同时进行 SDS 降解和生物表面活性剂合成的铜绿假单胞菌 S15。使用十六烷基三甲基溴化铵-亚甲蓝方法在 SDS 基本琼脂平板上证明了基于 SDS 的鼠李糖脂生产，并在基于 SDS 的基本盐 (SBS) 培养基中进行了优化。 SDS 被证明是鼠李糖脂合成的理想碳源，具有较高的底物到产物的转化率，5 天内从 1 g/l SDS 产生 6.9 g/l 鼠李糖脂。对纯化的生物表面活性剂进行快原子轰击质谱分析，证明存在单鼠李糖脂和二鼠李糖脂，即具有表面活性的 Rha-C-10-C-10、Rha-C-10-C-12 和 Rha-Rha-C-10-C-10。 S15 不利用分泌的鼠李糖脂作为碳源，但它导致细菌生物膜在 SBS 培养基中分散。据我们所知，这是第一份关于合成洗涤剂生物转化为生物洗涤剂的报告。这种基于 SDS 的新颖方法提出了一种利用 SDS 作为唯一碳源的更经济的鼠李糖脂合成模式。
The utility of rhamnolipids in industry is currently limited due to the high constraints in its economic production. In this scenario, the novel utility of sodium dodecyl sulphate (SDS) as carbon source could serve as promising cost-effective strategy. Screening of effective SDS biodegraders led to the isolation of Pseudomonas aeruginosa S15 capable of concomitant SDS degradation and biosurfactant synthesis. SDS-based rhamnolipid production was proved on SDS minimal agar plates using cetyl trimethylammonium bromide-methylene blue method and optimised in SDS-based minimal salt (SBS) medium. SDS proved to be an ideal carbon source for rhamnolipid synthesis with a high substrate to product conversion rate yielding 6.9 g/l of rhamnolipids from 1 g/l SDS in 5 days. Fast atom bombardment mass spectroscopy analysis of the purified biosurfactant proved the presence of mono- and di-rhamnolipids, viz., Rha-C-10-C-10, Rha-C-10-C-12 and Rha-Rha-C-10-C-10 with surface active properties. The secreted rhamnolipids were not utilised by S15 as a carbon source, but it caused a dispersion of bacterial biofilms in SBS medium. To the best of our knowledge, this is the first report on bioconversion of synthetic detergent to biodetergent. This SDS-based novel methodology presents a more economised mode of rhamnolipid synthesis utilising SDS as sole carbon source.