Co-expression of Oct-4 and Nestin in human breast cancers
Co-expression of Oct-4 and Nestin in human breast cancers
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Oct-4 和 Nestin 在人类乳腺癌中的共表达
DOI：
10.1007/s11033-011-1398-6
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发表时间：
2012-05-01
影响因子：
2.8
通讯作者：
Jin, Feng
中科院分区：
文献类型：
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作者：
Liu, Caigang;Cao, Xuezhao;Jin, Feng
目的是研究Oct-4和Nestin蛋白在人类乳腺癌中的临床意义。选取346例（26例新鲜肿瘤组织，320例石蜡包埋肿瘤组织），采用流式细胞术检测CD 44（+）CD 24（-）肿瘤细胞的频率，并采用PCR芯片和免疫荧光技术分析CD 44（+）CD 24（-）和非CD 44（+）CD 24（-）肿瘤细胞干细胞相关基因的差异表达。与非CD 44（+）CD 24（-）肿瘤细胞相比，CD 44（+）CD 24（-）肿瘤细胞，尤其是Oct-4（+）和Nestin（+）高比例的肿瘤细胞，通过体外形成乳腺球，具有更高的致瘤性。在320例肿瘤组织中，Oct-4和Nestin染色阳性42例（13.125%）。多因素分析显示，Oct-4和Nestin的表达与患者年龄、病理分级、淋巴结转移、三阴性乳腺癌相关（P < 0.05），与患者生存期相关（P = 0.001）。Oct-4和Nestin是乳腺癌发生发展的重要调节因子，Oct-4和Nestin可作为乳腺癌预后的预测指标。
The aim is to investigate the clinical implications of the Oct-4 and Nestin protein in human breast cancers. A total of 346 cases including 26 fresh and 320 paraffin-embedded tumor tissues were selected for characterizing the frequency of CD44(+)CD24(-) tumor cells by flow cytometry and the differential expression of the stem cell-related genes between CD44(+)CD24(-) and non-CD44(+)CD24(-) tumor cells was analyzed by PCR Array and immunofluorescence. In comparison with the non-CD44(+)CD24(-) tumor cells, the CD44(+)CD24(-), particularly for those with high percentage of Oct-4(+) and Nestin(+), tumor cells had higher tumorigenicity by forming mammospheres in vitro. More importantly, 42 (13.125%) out of 320 tumor tissues were positive for Oct-4 and Nestin staining. Universal analysis and multivariate analysis revealed that the expression of Oct-4 and Nestin was associated significantly with younger age, pathogenic degrees, lymph node metastasis and triple-negative breast cancer independently (P < 0.05) as well as shorter survival (P = 0.001). Oct-4 and Nestin were important regulators of the development of breast cancer, and Oct-4 and Nestin may be used as predictors for the prognosis of breast cancers.