CD34 expression by stromal precursors in normal human adult bone marrow.

CD34 expression by stromal precursors in normal human adult bone marrow.
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DOI：
10.1182/blood.v78.11.2848.2848
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发表时间：
1991-12
期刊：
Blood
影响因子：
20.3
通讯作者：
P. Simmons;B. Torok-Storb;Fred Hutchin
P. Simmons;B. Torok-Storb;Fred Hutchin
中科院分区：
医学1区
文献类型：
--
作者：
P. Simmons;B. Torok-Storb;Fred Hutchin

文献摘要

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在CD34抗原表达的基础上，用荧光激活细胞分选法(FACS)分离正常骨髓细胞，然后在体外检测成纤维细胞集落(成纤维细胞集落形成单位[CFU-F])。在CD34+人群中，超过95%的可检测CFU-F被恢复，而在CD34-人群中，它们的数量明显减少。进一步的实验表明，大多数CFU-F表现出高度的正向和垂直光散射以及低密度的CD34抗原。分选细胞在长期骨髓培养(LTMC)优化的培养基中生长，产生一种复杂的黏附基质元素混合物，包括成纤维细胞、脂肪细胞、平滑肌细胞和巨噬细胞。识别骨髓基质细胞的单抗Stro-1可与约5%的CD34+细胞反应，包括LTMC中的所有CFU-F和基质前体细胞。使用大豆凝集素(SBA)的实验进一步表明，这些基质元素仅限于表型为CD34+/SBA+的骨髓细胞群。基质前体的这些特性与原始造血祖细胞的特性非常不同，表明尽管造血和基质系统的前体共同表达CD34，但它们在其他方面是不同的表型。
Normal bone marrow cells were isolated by fluorescence-activated cell sorting (FACS) on the basis of CD34 antigen expression and then assayed in vitro for colonies of fibroblastic cells (fibroblast colony-forming units [CFU-F]). Greater than 95% of detectable CFU-F were recovered in the CD34+ population, while their numbers were markedly depleted in the CD34- population. Additional experiments showed that the majority of CFU-F exhibited high forward and perpendicular light scatter and low-density CD34 antigen. Growth of sorted cells in medium optimized for long-term marrow culture (LTMC) produced a complex mixture of adherent stromal elements including fibroblasts, adipocytes, smooth muscle cells, and macrophages. Monoclonal antibody STRO-1, which identifies bone marrow stromal cells, reacted with approximately 5% of CD34+ cells, which included all CFU-F and stromal precursors in LTMC. Experiments using soybean agglutinin (SBA) further showed that these stromal elements were restricted to a population of bone marrow cells with the phenotype CD34+/SBA+. These properties of stromal precursors are quite distinct from those of primitive hematopoietic progenitors, showing that although the precursors of the hematopoietic and stromal systems share expression of CD34, they are otherwise phenotypically distinct cell types.