Novel Acute Retinal Artery Ischemia and Reperfusion Model in Nonhuman Primates
Novel Acute Retinal Artery Ischemia and Reperfusion Model in Nonhuman Primates
复制标题
非人类灵长类动物的新型急性视网膜动脉缺血和再灌注模型
DOI：
10.1161/strokeaha.119.028809
复制
发表时间：
2020-08-01
期刊：
影响因子：
8.3
通讯作者：
Zhang, Xuxiang
中科院分区：
文献类型：
--
作者：
Gao, Yuan;Wu, Di;Zhang, Xuxiang
补充数字内容可在文本中找到。背景和目的：视网膜作为位于外部的神经组织，在研究治疗干预对大脑的影响方面具有独特的优势。在这项研究中，我们提出了一个临床相关的模型，视网膜缺血和再灌注在非人灵长类动物。研究方法：通过将自体凝块注射到成年恒河猴的眼动脉中来诱导急性视网膜动脉缺血和再灌注，并通过用tPA（组织型纤溶酶原激活剂）进行局部溶栓来实现再通。数字减影血管造影和荧光素血管造影用于评估视网膜和脉络膜的血流。采用视网膜电图、光学相干断层扫描、苏木精-伊红染色等方法评价缺血后视网膜的结构和功能。结果如下：数字减影血管造影和荧光素血管造影图像证实眼动脉和视网膜中央动脉闭塞，以及tPA溶栓后再通。视网膜电图显示缺血后视网膜功能受损，溶栓可部分挽救其受损。光学相干断层扫描和苏木精-伊红染色显示缺血引起的视网膜变化，tPA部分减轻这些损害。结论：这种新的恒河猴急性视网膜动脉缺血再灌注模型可以很好地模拟临床上的视网膜缺血再灌注，并为筛选神经保护策略提供最佳平台。
Supplemental Digital Content is available in the text. Background and Purpose: The retina, as an externally located neural tissue, offers unique advantages in investigating the effect of therapeutic intervention on the brain. In this study, we put forth a clinically relevant model of retinal ischemia and reperfusion in nonhuman primates. Methods: Acute retinal artery ischemia and reperfusion was induced by injecting an autologous clot into the ophthalmic artery of adult rhesus monkeys, and recanalization was achieved by focal thrombolysis with tPA (tissue-type plasminogen activator). Digital subtraction angiography and fluorescein angiography were used to evaluate blood flow in the retina and the choroid. Electroretinogram, optical coherence tomography, and hematoxylin and eosin staining were used to evaluate the structure and function of the retina after ischemia. Results: Digital subtraction angiography and fluorescein angiography images confirmed occlusion of the ophthalmic and central retinal arteries, as well as recanalization after tPA thrombolysis. Electroretinogram indicated retinal functional damage following ischemia, and thrombolysis partially rescued its impairment. Optical coherence tomography and hematoxylin and eosin staining revealed ischemia-induced changes in the retina, and tPA partially mitigated these damages. Conclusions: This novel acute retinal artery ischemia and reperfusion model in rhesus monkeys may closely simulate retinal ischemia/reperfusion in clinical practice and provide an optimal platform for screening neuroprotective strategies.