Expression of the Full-Length and 3′-Spliced cry1Ab Gene in the 135-kDa Crystal Protein Minus Derivative of Bacillus thuringiensis subsp. kyushuensis
Expression of the Full-Length and 3′-Spliced cry1Ab Gene in the 135-kDa Crystal Protein Minus Derivative of Bacillus thuringiensis subsp. kyushuensis
复制标题
苏云金芽孢杆菌九州亚种 135 kDa 晶体蛋白减去衍生物中全长和 3′ 剪接的 cry1Ab 基因的表达。
DOI：
10.1007/s00284-001-0092-7
复制
发表时间：
2002
影响因子：
2.6
通讯作者：
Y. Pang
中科院分区：
文献类型：
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作者：
Jianxiu Yu;Ruiyu Xie;Le Tan;W. Xu;Shao;Jian;M. Tang;Y. Pang
苏云金芽孢杆菌（Bacillus thuringiensis）产生一种分子量为130-135-kDa的杀虫蛋白，其晶体呈双锥状，对鳞翅目幼虫具有毒性。将天然Cry 1Ab的C端部分序列替换为Cry 11 Aa的C端异源序列，得到3′-剪接的cry 1Ab基因。将全长cry 1Ab和3′-剪接的cry 1Ab克隆到E.大肠杆菌苏云金杆菌穿梭表达载体pHZB 1，在135-kDa的晶体蛋白中表达B.苏云金杆菌九州亚种（4U 1-Cry−135）。两种重组体的Cry 1Ab蛋白的晶体形状均为规则的双锥体，而完整的Cry 1Ab的晶体尺寸约为3′-剪接的Cry 1Ab的5倍。此外，两种Cry 1Ab蛋白对银纹夜蛾的毒力相似。
Bacillus thuringiensisproduces a 130–135-kDa insecticidal protein in the form of bipyramidal crystal which is toxic to lepidopteran larvae. Part of the C-terminal region of the native Cry1Ab was replaced by a heterologous sequence of Cry11Aa C-terminus to get a 3′-splicedcry1Ab gene. The full-lengthcry1Ab and 3′-splicedcry1Ab, which were both cloned into theE. coli–B. thuringiensisshuttle expression vector pHZB1, were expressed in a 135-kDa crystal protein minus derivative ofB. thuringiensissubsp.kyushuensis(4U1-Cry−135). The crystal shape of Cry1Ab proteins from both recombinants was regularly bipyramidal, while the crystal size of the intact Cry1Ab was approximately fivefold larger than the 3′-spliced Cry1Ab. In addition, these two kinds of Cry1Ab proteins had similar toxicity againstArgyrogramma agnatalarvae.
过氧化氢光解灭菌法对蜡样芽孢杆菌芽孢的杀芽作用
DOI：
--
发表时间：
2022
期刊：
影响因子：
--
作者：
宍戸駿一;白土翠;倉内美智子;中村圭祐;菅野太郎;庭野吉己
通讯作者：
庭野吉己