Biomass measurement by inductive permittivity.
Biomass measurement by inductive permittivity.
复制标题
通过感应介电常数测量生物量。
DOI：
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发表时间：
1997
影响因子：
3.8
通讯作者：
S. Siano
中科院分区：
文献类型：
--
作者：
S. Siano
无电极感应介电常数探头能够可靠地指示发酵生物量。通过基于电磁感应设计探头，可以消除电极极化伪影（导电溶液基于电极的介电常数测量中常见的现象）。活生物量浓度 (X(V)) 的测量精确且线性，干扰可忽略不计，如两种细菌、两种酵母和两种哺乳动物细胞所示。介电常数谱通常可以通过 Cole-Cole 方程的三个参数来描述：最大（低频）介电常数、特征频率 (f(C)) 和 Cole-Cole α。 f(C) 和 alpha 具有分别作为细胞大小和形态的相对指标的潜力。 (c) 1997 年 John Wiley & Sons, Inc. Biotechnol Bioeng 55: 289-304, 1997。
An electrodeless, inductive permittivity probe is shown to reliably indicate fermentation biomass. By designing the probe based on electromagnetic induction, the electrode polarization artifact-common to electrode-based permittivity measurements of conductive solutions-is eliminated. The measurement of viable biomass concentration (X(V)) is precise and linear, with negligible interference, as shown for two bacteria, two yeasts, and two mammalian cells. The permittivity spectra often can be described by the three parameters of the Cole-Cole equation: the maximum (low-frequency) permittivity, the characteristic frequency (f(C)), and the Cole-Cole alpha. The f(C) and alpha have potential as relative indicators of cell size and morphology, respectively. (c) 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 55: 289-304, 1997.