Changes in mouse serum proteins during acute and chronic infection with an intracellular parasite (Toxoplasma gondii).
Changes in mouse serum proteins during acute and chronic infection with an intracellular parasite (Toxoplasma gondii).
复制标题
细胞内寄生虫（弓形虫）急性和慢性感染期间小鼠血清蛋白的变化。
DOI：
10.4049/jimmunol.95.6.1023
复制
发表时间：
1965
影响因子：
4.4
通讯作者：
R. Hackman
中科院分区：
文献类型：
--
作者：
J. Remington;R. Hackman
在弓形虫强毒株和相对强毒株感染急性期和慢性期过程中，小鼠血清蛋白的电泳谱和免疫电泳谱发生了显着变化。在感染强毒RH株的小鼠中，α-和β-球蛋白显著增加，白蛋白减少。血清和腹腔液免疫电泳谱显示，结合珠蛋白显著增加，β1C向β1D转化，两个主要的β球蛋白沉淀弧消失，白蛋白迁移率增加。在一些感染了毒力较低的菌株的动物群体中，也注意到了类似但不太明显的变化。在感染M7741和H44菌株的小鼠中，出现了“慢性”高丙种球蛋白血症。用原始无毒株或RH株攻击慢性感染的小鼠，在大多数动物的血清学试验效价没有显著上升的情况下，导致非常高的γ-球蛋白水平。这种血清与增殖生物的吸附导致抗体消失或显著减少，但对γ球蛋白水平没有明显影响。
这些发现与动物和人类的慢性感染有关，并提出了一种假设，即存在一种形式的寄生虫，而不是包囊，以解释持久抗体形成、高丙种球蛋白血症和寄生虫血症。
Electrophoretic and immunoelectrophoretic patterns of mouse serum proteins are markedly altered during the course of the acute and chronic stages of infection with virulent and relatively avirulent strains of Toxoplasma gondii. In mice infected with the virulent RH strain, there was a remarkable increase in α- and β-globulins and decrease in albumin. Immunoelectrophoretic patterns of serum and peritoneal fluid of these animals revealed a marked increase in haptoglobin, conversion of β1C to β1D, disappearance of two major β-globulin precipitin arcs, and an increase in mobility of albumin. Similar but less marked changes were noted in some of the groups of animals infected with less virulent strains. In mice infected with strains M7741 and H44 a state of “chronic” hypergammaglobulinemia developed. Challenge of chronically infected mice with the original avirulent strain or with the RH strain resulted in very high levels of γ-globulins in the absence, in most animals, of significant rises in serologic test titers. Adsorption of such sera with proliferative organisms resulted in disappearance or marked decrease in antibodies but had no demonstrable effect on γ-globulin levels.
These findings are discussed in relation to the chronic infection in animals and humans, and an hypothesis is posed suggesting the existence of a form of the parasite other than the cyst to account for persistent antibody formation, hypergamma-globulinemia and parasitemia.