Mortalin expression in pancreatic cancer and its clinical and prognostic significance
Mortalin expression in pancreatic cancer and its clinical and prognostic significance
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Mortalin在胰腺癌中的表达及其临床和预后意义
DOI：
10.1016/j.humpath.2017.03.015
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发表时间：
2017-06-01
期刊：
影响因子：
3.3
通讯作者：
Jin, Aihua
中科院分区：
文献类型：
--
作者：
Cui, Xuelian;Li, Zhenling;Jin, Aihua
莫他林，一种必不可少的线粒体！伴侣蛋白，与多种恶性肿瘤的发生有关。本研究旨在研究Mortalin在胰腺导管腺癌(PDAC)细胞中的表达，并探讨其临床病理和预后意义。用免疫荧光法检测Mortalin蛋白在BXPC-3PDAC细胞中的定位。应用免疫组织化学方法检测106例PDAC患者和46例相应的非肿瘤胰腺组织中Mortalin的表达。收集临床病理参数和总体生存数据，并比较不同Mortalin状态之间的差异。免疫组织化学和免疫荧光结果显示，Mortalin主要存在于PDAC细胞的胞浆中。在PDAC组织中，Mortalin强阳性表达率(55.66%；59/106)明显高于癌旁正常组织(23.91%；11/46)。Mortalin的表达与临床分期、神经侵袭、淋巴结转移和总体生存率呈正相关。多变量COX回归分析表明，除位置、临床分期和神经侵袭外，莫他林是影响PDAC患者生存的重要独立预后因素。因此，我们提出了强有力的证据表明，莫他林可以作为预测预后的实用标记物，并作为PDAC治疗的潜在治疗靶点。(C)2017 Elsevier Inc.保留所有权利。
Mortalin, an essential mitochondria! chaperone protein, is involved in the tumorigenesis of a number of malignancies. This study aimed to investigate the expression of Mortalin in pancreatic ductal adenocarcinoma (PDAC) cells and to determine its clinicopathological and prognostic significance. The localization of Mortalin protein was detected in BXPC-3 PDAC cells using immunofluorescence. Immunohistochemistry was also used to detect Mortalin expression in well-defined tissues obtained from 106 PDAC patients and 46 corresponding nontumor pancreatic tissues. Clinicopathological parameters and overall survival data were collected and compared between different Mortalin statuses. The results of immunohistochemistry and immunofluorescence showed that Mortalin was primarily present in the cytoplasm of PDAC cells. The ratio of strong positive staining for Mortalin was higher in PDAC tissues (55.66%; 59/106) than in normal adjacent tissues (23.91%; 11/46). Positive relationships between Mortalin expression and clinical stage, perineural invasion, lymph node metastasis, and lower overall survival were observed. Multivariate Cox regression analysis identified Mortalin as a significant independent prognostic factor, in addition to location, clinical stage, and perineural invasion, for survival of PDAC patients. Therefore, we present strong evidence that Mortalin may function as a practical marker to predict prognosis and as a potential therapeutic target in PDAC treatment. (C) 2017 Elsevier Inc. All rights reserved.