FTIR microscopy as a method for identification of bacterial and fungal infections
FTIR microscopy as a method for identification of bacterial and fungal infections
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DOI：
10.1016/j.jpba.2004.08.010
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发表时间：
2005-04-29
影响因子：
3.4
通讯作者：
Huleihel, M
中科院分区：
文献类型：
--
作者：
Erukhimovitch, V;Pavlov, V;Huleihel, M
傅里叶变换红外（FTIR）显微镜被认为是一种全面和灵敏的方法，用于检测细胞中的分子变化。FTIR显微光谱法相对于传统FTIR光谱法的优势在于，它便于检查被检样品的受限区域。在本研究中，我们研究了FTIR显微镜作为一种简单，快速和可靠的技术，用于区分细菌和真菌，这两者都涉及各种人类和其他动物感染的潜力。在许多情况下，没有简单快速的技术来确定这种感染的原因，无论是细菌，真菌还是两者兼而有之。快速了解这些信息对于有效治疗非常重要。我们的研究结果证明了细菌和真菌样品之间可检测到的显著光谱差异。在细菌和真菌混合物的光谱中，细菌和真菌的代表峰清晰地出现。这种技术似乎可以用于快速区分细菌和真菌感染和污染。(c)2004 Elsevier B.V.保留所有权利。
Fourier-transform infrared (FTIR) microscopy is considered to be a comprehensive and sensitive method for detection of molecular changes in cells. The advantage of FTIR microspectroscopy over conventional FTIR spectroscopy is that it facilitates inspection of restricted regions of the examined sample. In the present study, we examined the potential of FTIR microscopy as an easy, rapid and reliable technique for discrimination between bacteria and fungi both of which are involved in various human and other animal infections. In many cases, there is no easy and rapid technique for identifying the cause of such infections whether it is bacteria, fungi or both. Knowing such information in a rapid way could be highly important for effective therapy. Our results proved detectable and significant spectral differences between bacterial and fungal samples. Representative peaks of bacteria and fungi appeared clearly in the spectra of a mixture of bacteria and fungi. It seems that this technique could be used for rapid discrimination between bacterial and fungal infections and contaminations. (c) 2004 Elsevier B.V. All rights reserved.