Quantitative Cerebral Blood Flow Measurements in the Rat Using a Beta-Probe and H215O
Quantitative Cerebral Blood Flow Measurements in the Rat Using a Beta-Probe and H215O
复制标题
使用 Beta 探针和 H215O 定量测量大鼠脑血流量
DOI：
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发表时间：
2003
影响因子：
6.3
通讯作者：
A. Buck
中科院分区：
文献类型：
--
作者：
B. Weber;N. Spaeth;M. Wyss;D. Wild;C. Burger;Ross Stanley;A. Buck
-探针是一种相对较新的动物示踪动力学研究工具。它们非常适合评估新的正电子发射断层扫描示踪剂或测量静息时或经过某种刺激或干预后的生理参数。在许多这样的实验中，CBF的知识是非常重要的。因此，本研究的目的是评估使用β -探针和H215O测量CBF的方法。测定了8只大鼠在基线和乙酰唑胺刺激后的脑皮质CBF。另外对5只动物进行三叉神经刺激。在每个类别中，每隔10分钟注射三次250至300 MBq的H215O。使用标准的单组织室模型（K1 = CBF, k2 = CBF/p，其中p为分区系数）分析数据。静息、乙酰唑胺刺激和三叉神经刺激时K1值分别为0.35±0.09、0.58±0.16和0.49±0.03 mL·min−1·mL−1。k2的对应值分别为0.55±0.12、0.94±0.16和0.85±0.12 min−7，p的对应值分别为0.64±0.05、0.61±0.07和0.59±0.06。对于K1、k2和p，两个连续实验之间的差异的标准偏差分别为10.1%、13.0%和5.7%，这是衡量方法重复性的一个指标。总之，β -探针与H215O结合使用可以对CBF进行可重复的定量测量，尽管可能由于部分体积效应，可能会出现一些系统性的低估。
Beta-probes are a relatively new tool for tracer kinetic studies in animals. They are highly suited to evaluate new positron emission tomography tracers or measure physiologic parameters at rest and after some kind of stimulation or intervention. In many of these experiments, the knowledge of CBF is highly important. Thus, the purpose of this study was to evaluate the method of CBF measurements using a beta-probe and H215O. CBF was measured in the barrel cortex of eight rats at baseline and after acetazolamide challenge. Trigeminal nerve stimulation was additionally performed in five animals. In each category, three injections of 250 to 300 MBq H215O were performed at 10-minute intervals. Data were analyzed using a standard one-tissue compartment model (K1 = CBF, k2 = CBF/p, where p is the partition coefficient). Values for K1 were 0.35 ± 0.09, 0.58 ± 0.16, and 0.49 ± 0.03 mL · min−1 · mL−1 at rest, after acetazolamide challenge, and during trigeminal nerve stimulation, respectively. The corresponding values for k2 were 0.55 ± 0.12, 0.94 ± 0.16, and 0.85 ± 0.12 min−7, and for p were 0.64 ± 0.05, 0.61 ± 0.07, and 0.59 ± 0.06. The standard deviation of the difference between two successive experiments, a measure for the reproducibility of the method, was 10.1%, 13.0%, and 5.7% for K1, k2, and p, respectively. In summary, beta-probes in conjunction with H215O allow the reproducible quantitative measurement of CBF, although some systematic underestimation seems to occur, probably because of partial volume effects.
DOI：
10.1016/0167-8760(95)00042-9
发表时间：
1996
期刊：
International journal of psychophysiology : official journal of the International Organization of Psychophysiology
影响因子：
--
作者：
Y. Moskalenko;J. Dowling;D. Liu;C. Rovainen;V. N. Semernia;T. Woolsey
通讯作者：
Y. Moskalenko;J. Dowling;D. Liu;C. Rovainen;V. N. Semernia;T. Woolsey