Osteogenic matrix sheet-cell transplantation using osteobtastic cell sheet resulted in bone formation without scaffold at an ectopic site
Osteogenic matrix sheet-cell transplantation using osteobtastic cell sheet resulted in bone formation without scaffold at an ectopic site
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DOI：
10.1002/term.81
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发表时间：
2008-06-01
影响因子：
3.3
通讯作者：
Takakura, Yoshinori
中科院分区：
文献类型：
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作者：
Akahane, Manabu;Nakamura, Akifumi;Takakura, Yoshinori
我们之前报道过，可以在多孔羟基磷灰石（HA）支架和培养的间充质干细胞（MSC）的复合材料中观察到体内骨形成。在本研究中，我们开发了一种移植培养的间充质干细胞的新方法，无需使用支架形成骨组织。 MSC 被培养扩增并提升为细胞片结构。这些细胞片被称为成骨基质片，显示出阳性碱性磷酸酶（ALP）染色、高ALP活性和高骨钙素（OC）含量，表明它们的成骨潜力。我们将这些片材移植到大鼠的皮下部位，以评估它们是否具有体内成骨能力。移植后 6 周，移植的片材显示出矿化基质、骨细胞和活跃的成骨细胞衬里，表明新骨形成。 HA支架也用片材包裹以制成HA/片材复合材料并植入大鼠的皮下部位。复合材料的组织学切片显示植入后 4 周，HA 孔中出现骨形成。我们目前的结果表明，间充质干细胞可以作为片状结构进行培养，所得的片状本身或HA-片状复合材料代表了可用于硬组织重建的成骨植入物。版权所有 (C) 2008 约翰·威利父子有限公司
We previously reported that in vivo bone formation could be observed in composites of porous hydroxyapatite (HA) scaffolds and cultured mesenchymal stem cells (MSCs). In the present study, we developed a new method for transplantation of cultured MSCs without the necessity of using a scaffold to form bone tissue. MSCs were culture-expanded and lifted as cell sheet structures. These cell sheets, designated osteogenic matrix sheets, showed positive alkaline phosphatase (ALP) staining, high ALP activities and high osteocalcin (OC) contents, indicating their osteogenic potential. We transplanted these sheets into subcutaneous sites in rats to assess whether they possessed in vivo bone-forming capability. The transplanted sheets showed mineralized matrix together with osteocytes and an active osteoblast lining, indicating new bone formation, at 6 weeks after transplantation. HA scaffolds were also wrapped with the sheets to make HA/sheet composites and implanted into subcutaneous sites in rats. Histological sections of the composites revealed bone formation in the HA pores at 4 weeks after implantation. Our present results indicate that MSCs can be cultured as sheet structures, and the resulting sheets themselves or HA-sheet composites represent osteogenic implants that can be used for hard tissue reconstruction. Copyright (C) 2008 John Wiley & Sons, Ltd.