Graphene Oxide-Based Amplified Fluorescent Biosensor for Hg2+ Detection through Hybridization Chain Reactions
Graphene Oxide-Based Amplified Fluorescent Biosensor for Hg2+ Detection through Hybridization Chain Reactions
复制标题
DOI：
10.1021/ac500192r
复制
发表时间：
2014-03-18
影响因子：
7.4
通讯作者：
Li, Zhigang
中科院分区：
文献类型：
--
作者：
Huang, Jiahao;Gao, Xiang;Li, Zhigang
我们报道了一种基于氧化石墨烯（GO）的荧光传感器，通过杂交链反应（HCR）检测水溶液中的Hg 2+。GO被用作捕获单链DNA的吸附材料和用于降低背景信号的有效荧光猝灭剂。在检测策略中，使用两个发夹探针和辅助DNA。在没有Hg 2+的情况下，它们被GO吸附，并且发夹探针之一的荧光被淬灭。在Hg ~（2+）存在下，两个发夹探针之间的HCR由Hg ~（2+）在辅助DNA的帮助下通过T-Hg ~（2+）-T配位化学引发。HCR的双链DNA产物被GO释放，荧光被恢复。传感方法的检测限为0.3 nM，这对于实际应用是足够灵敏的。该传感系统对其他二价金属离子也表现出较高的选择性，该传感器在饮用水中的应用表明，该方法对真实的样品效果良好。
We report a graphene oxide (GO)-based fluorescent sensor for Hg2+ detection in aqueous solutions by using hybridization chain reactions (HCRs). GO is used as an adsorption material for capturing single-stranded DNA and an efficient fluorescence quencher for reducing the background signal. In the detection strategy, two hairpin probes and a helper DNA are employed. Without Hg2+, they are adsorbed by the GO and the fluorescence of one of the hairpin probes is quenched. In the presence of Hg2+, the HCRs between the two hairpin probes are initiated by Hg2+ with the aid of the helper DNA through T-Hg2+-T coordination chemistry. The double-stranded DNA products of the HCRs are released by the GO and the fluorescence is recovered. The detection limit of the sensing method is 0.3 nM, which is sufficiently sensitive for practical applications. The sensing system also exhibits high selectivity against other divalent metal ions, and the application of the sensor for drinking water shows that the proposed method works well for real samples.