Bone Apatite Composition of Necrotic Trabecular Bone in the Femoral Head of Immature Piglets
Bone Apatite Composition of Necrotic Trabecular Bone in the Femoral Head of Immature Piglets
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DOI：
10.1007/s00223-015-9959-7
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发表时间：
2015-04-01
影响因子：
4.2
通讯作者：
Aswath, Pranesh B.
中科院分区：
文献类型：
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作者：
Aruwajoye, Olumide O.;Kim, Harry K. W.;Aswath, Pranesh B.
股骨头缺血性坏死(IOFH)可导致骨小梁过度吸收和股骨头整体塌陷。骨小梁的一种众所周知的矿物成分是羟基磷灰石，由于离子取代，它可以以多种形式存在，从而改变化学成分。不幸的是，人们对IOFH后骨磷灰石的化学变化知之甚少。我们推测，坏死骨中磷灰石成分的变化可能是导致破骨细胞吸收增加和股骨头结构性塌陷的原因。这项研究的目的是评估活跃吸收的坏死骨小梁的宏观和局部磷酸盐成分，以区分破骨细胞吸收增加的区域和正常骨形成区域之间的差异。采用仔猪IOFH模型。对股骨头进行扫描电子显微镜(SEM)、组织学、X射线近缘结构(XANES)和拉曼光谱分析，以表征正常和坏死的松质骨。背向散射扫描、计算机断层扫描和组织学检查显示，与正常相比，坏死骨畸形和活跃的吸收。从活动性吸收的坏死骨和正常骨中获得的XANES和拉曼光谱显示，坏死骨中碳酸盐与磷酸盐的含量增加。碳酸盐替代引起的磷灰石成分的变化可能在坏死骨的吸收增加中起作用，因为它的溶解度增加。事实上，更好地了解坏死骨的磷灰石成分可以揭示破骨细胞的活动，并有可能改进针对骨过度吸收的治疗方法。
Ischemic osteonecrosis of the femoral head (IOFH) can lead to excessive resorption of the trabecular bone and collapse of the femoral head as a structure. A well-known mineral component to trabecular bone is hydroxyapatite, which can be present in many forms due to ionic substitution, thus altering chemical composition. Unfortunately, very little is known about the chemical changes to bone apatite following IOFH. We hypothesized that the apatite composition changes in necrotic bone possibly contribute to increased osteoclast resorption and structural collapse of the femoral head. The purpose of this study was to assess the macroscopic and local phosphate composition of actively resorbed necrotic trabecular bone to isolate differences between areas of increased osteoclast resorption and normal bone formation. A piglet model of IOFH was used. Scanning electron microscopy (SEM), histology, X-ray absorbance near edge structure (XANES), and Raman spectroscopy were performed on femoral heads to characterize normal and necrotic trabecular bone. Backscattered SEM, micro-computed tomography and histology showed deformity and active resorption of necrotic bone compared to normal. XANES and Raman spectroscopy obtained from actively resorbed necrotic bone and normal bone showed increased carbonate-to-phosphate content in the necrotic bone. The changes in the apatite composition due to carbonate substitution may play a role in the increased resorption of necrotic bone due to its increase in solubility. Indeed, a better understanding of the apatite composition of necrotic bone could shed light on osteoclast activity and potentially improve therapeutic treatments that target excessive resorption of bone.