Analysis of human papillomavirus prevalence and TP53 polymorphism in head and neck squamous cell carcinomas
Analysis of human papillomavirus prevalence and TP53 polymorphism in head and neck squamous cell carcinomas
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DOI：
10.1016/j.cancergencyto.2003.07.010
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发表时间：
2004-04-01
影响因子：
--
通讯作者：
Rahal, P
中科院分区：
文献类型：
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作者：
Cortezzi, SS;Provazzi, PJ;Rahal, P
头颈部鳞状细胞癌是一种与烟草和酒精滥用有关的疾病。有证据表明，致癌的人乳头瘤病毒（HPV）也可能是上呼吸消化道癌症的风险。高危型HPV编码两种早期蛋白E6和E7，它们可以分别与p53和pRb结合，并诱导其降解或失活。TP 53基因在外显子4的第72位密码子处具有单一多态性，其编码精氨酸（Arg）或脯氨酸（Pro）。本研究旨在探讨HPV感染和TP 53基因多态性在头颈部肿瘤中的作用。我们用聚合酶链反应技术分析了50个肿瘤，以及142个对照个体的口腔粘膜拭子。对照组中HPV的患病率为10.6%，癌症标本中为16%。对照组基因型频率分布为Arg/Arg 50%，Arg/ Pro 43%，Pro/Pro 7%，肿瘤组基因型频率分布为Arg/Arg 52%，Arg/Pro 32%，Pro/Pro 16%。与一些宫颈癌研究的结果相反，没有观察到任何TP 53基因型或等位基因与头颈癌的发展之间的关联，无论HPV状态如何，除了Pro/Pro基因型，其与HPV的缺乏相关。精氨酸等位基因似乎可以预防头颈癌。此外，数据显示，HPV感染不会增加患头颈部肿瘤的风险。(C)2004年爱思唯尔公司All rights reserved.
Head and neck squamous cell carcinoma is a disease associated with tobacco and alcohol abuse. There is evidence that the oncogenic human papillomavirus (HPV) may also be a risk for upper aerodigestive tract cancers. High-risk HPVs encode two early proteins, E6 and E7, that can bind to p53 and pRb, respectively, and induce its degradation or inactivation. The TP53 gene has a single polymorphism at codon 72 of exon 4 that encodes either arginine (Arg) or proline (Pro). The purpose of this study was to evaluate the role of HPV infection and TP53 polymorphism in head and neck cancer. We analyzed 50 tumors, as well swabs of oral mucosa front 142 control individuals, with a polymerase chain reaction technique. The prevalence of HPV in controls was 10.6% and in cancer specimens 16%. The frequency distribution of genotypes in controls was 50% Arg/Arg, 43% Arg/ Pro and 7% Pro/Pro; in tumors, it was 52% Arg/Arg, 32% Arg/Pro, and 16% Pro/Pro. Contrary to the results of some studies on cervical cancer, no association between any TP53 genotype or allele and the development of head and neck cancer was observed, regardless of HPV status, except for the Pro/Pro genotype, which is associated with the absence of HPV. The arginine allele appears to protect against head and neck cancers. Also, the data showed that HPV infection results in no increased risk of developing head and neck tumors. (C) 2004 Elsevier Inc. All rights reserved.