New technologies for examining the role of neuronal ensembles in drug addiction and fear.
New technologies for examining the role of neuronal ensembles in drug addiction and fear.
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DOI：
10.1038/nrn3597
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发表时间：
2013-11
期刊：
影响因子：
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通讯作者：
Hope BT
中科院分区：
文献类型：
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作者：
Cruz FC;Koya E;Guez-Barber DH;Bossert JM;Lupica CR;Shaham Y;Hope BT
相关数据表明，学习协会内的神经元合奏编码。然而，很难证明神经元集合介导学习行为，因为传统的药理学和损伤方法，甚至更新的细胞类型特异性方法，影响激活和非激活的神经元。此外，先前关于学习诱导的突触和分子改变的研究没有区分行为激活和非激活神经元。在这里，我们描述了三种新的方法Daun 02灭活，流式细胞仪分选的激活的神经元和c-fos-GFP转基因大鼠-已被用来选择性地针对和研究激活的神经元合奏模型的条件药物的影响和复发。我们还描述了两种新的工具- c-fos-tTA小鼠和CREB过表达神经元的失活-已被用于研究条件性恐惧中神经元集合的作用。
Correlational data suggest that learned associations are encoded within neuronal ensembles. However, it has been difficult to prove that neuronal ensembles mediate learned behaviours because traditional pharmacological and lesion methods, and even newer cell type-specific methods, affect both activated and non-activated neurons. Additionally, previous studies on synaptic and molecular alterations induced by learning did not distinguish between behaviourally activated and non-activated neurons. Here, we describe three new approaches—Daun02 inactivation, FACS sorting of activated neurons and c-fos-GFP transgenic rats — that have been used to selectively target and study activated neuronal ensembles in models of conditioned drug effects and relapse. We also describe two new tools — c-fos-tTA mice and inactivation of CREB-overexpressing neurons — that have been used to study the role of neuronal ensembles in conditioned fear.