Effects of Raloxifene on Ki-67 and CD34 Antigen Expression in Breast Cancer
Effects of Raloxifene on Ki-67 and CD34 Antigen Expression in Breast Cancer
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DOI：
10.1159/000165512
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发表时间：
2009-01-01
影响因子：
2.1
通讯作者：
Lopes-Costa, Pedro Vitor
中科院分区：
文献类型：
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作者：
da Silva, Benedito Borges;Pires, Cleicilene Gomes;Lopes-Costa, Pedro Vitor
背景：本研究的目的是评价雷洛昔芬对绝经后妇女乳腺癌标本中CD34和Ki-67抗原表达的影响。方法：16例绝经后可手术的II期乳腺癌患者，雌激素受体阳性，每天服用雷洛昔芬60 mg，共28天。采用免疫组织化学方法检测雷洛昔芬治疗前后肿瘤组织中CD34和Ki-67蛋白的表达。在每张载玻片上随机选择10个区域对血管生成进行量化，并使用图像捕获和分析系统对每张载玻片上的1000个细胞进行Ki-67染色的细胞核计数。放大。数据的统计分析采用配对样本的t检验。P<；0.05有统计学意义。结果：雷洛昔芬治疗前平均微血管数为44.44+/-3.54，治疗后为22.63+/-1.61(p<；0.001)，治疗前后Ki-67阳性细胞核的平均百分比分别为19.28+/-81.61和12.13+/-81.48(p<；0.001)。结论：雷洛昔芬可显著降低绝经后妇女乳腺癌组织中CD34和Ki-67蛋白的表达。版权所有(C)2008 S.Karger AG，巴塞尔
Background: The aim of this study was to evaluate the effect of raloxifene on CD34 and Ki-67 antigen expression in breast cancer specimens from postmenopausal women. Methods: Sixteen postmenopausal patients with operable, stage II (>= 3 cm), estrogen receptor-positive breast cancer, who took 60 mg of raloxifene daily for 28 days, participated in this study. Immunohistochemistry was carried out in tumor samples prior to and following raloxifene treatment to evaluate CD34 and Ki-67 protein expression. Angiogenesis was quantified in 10 randomly selected fields per slide, and Ki-67-stained nuclei were counted in 1,000 cells per slide using an image capture and analysis system with 400 ! magnification. Student's t test for paired samples was used for the statistical analysis of data. Statistical significance was established at p < 0.05. Results: The mean number of microvessels was 44.44 +/- 3.54 prior to raloxifene therapy and 22.63 +/- 1.61 following therapy (p < 0.001), and the mean percentage of Ki-67-stained nuclei was 19.28 +/- 8 1.61 and 12.13 +/- 8 1.48 prior to and following raloxifene treatment, respectively (p < 0.001). Conclusion: Raloxifene significantly reduces CD34 and Ki-67 protein expression in breast carcinoma in postmenopausal women. Copyright (C) 2008 S. Karger AG, Basel