Static and dynamic membrane properties of lateral vestibular nucleus neurons in guinea pig brain stem slices.
Static and dynamic membrane properties of lateral vestibular nucleus neurons in guinea pig brain stem slices.
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DOI：
10.1152/jn.00201.2003
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发表时间：
2003-09
影响因子：
2.5
通讯作者：
A. Uno;E. Idoux;M. Beraneck;P. Vidal;L. Moore;V. J. Wilson;N. Vibert
中科院分区：
文献类型：
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作者：
A. Uno;E. Idoux;M. Beraneck;P. Vidal;L. Moore;V. J. Wilson;N. Vibert
前庭中央神经元的体外细胞内记录迄今仅限于前庭内侧核（MVN）。我们对外侧前庭核（LVN）中的大deiter神经元进行了细胞内记录，以确定其静态和动态膜特性，并将其与MVN中识别的A型和B型神经元进行了比较。与MVN神经元（MVNn）不同，大尺寸LVN神经元（LVNn）形成均匀的细胞群，其特征是尖锐的尖峰，低振幅，双相超极化后的B型MVNn，以及a型MVNn。由于LVNn具有较低的膜电阻，在较大的频率范围内，LVNn对电流注入的敏感性低于MVNn。LVNn与MVNn的主要区别在于，LVNn灵敏度随刺激频率变化的波德图比MVNn更平坦，且共振更弱。此外，大多数LVNn在MVNn中观察到的频率范围内没有表现出发射速率调制的逐渐降低。LVNn的放电与高频正弦电流注入的去极化阶段同步。体内研究表明，MVN主要参与凝视控制，而投射到脊髓的巨大LVNn则参与姿势控制。我们认为LVNn和MVNn膜性质的差异可能反映了它们特定的生理作用。
In vitro intracellular recordings of central vestibular neurons have been restricted so far to the medial vestibular nucleus (MVN). We performed intracellular recordings of large Deiters' neurons in the lateral vestibular nucleus (LVN) to determine their static and dynamic membrane properties, and compare them with those of type A and type B neurons identified in the MVN. Unlike MVN neurons (MVNn), the giant-size LVN neurons (LVNn) form a homogeneous population of cells characterized by sharp spikes, a low-amplitude, biphasic after-hyperpolarization like type B MVNn, but also an A-like rectification like type A MVNn. In accordance with their lower membrane resistance, the sensitivity of LVNn to current injection was lower than that of MVNn over a large range of frequencies. The main difference between LVNn and MVNn was that the Bode plots showing the sensitivity of LVNn as a function of stimulation frequency were flatter than those of MVNn, and displayed a weaker resonance. Furthermore, most LVNn did not show a gradual decrease of their firing rate modulation in the frequency range where it was observed in MVNn. LVNn synchronized their firing with the depolarizing phase of high-frequency sinusoidal current injections. In vivo studies have shown that the MVN would be mainly involved in gaze control, whereas the giant LVNn that project to the spinal cord are involved in the control of posture. We suggest that the difference in the membrane properties of LVNn and MVNn may reflect their specific physiological roles.