Production of L-lactic acid from a mixture of xylose and glucose by co-cultivation of lactic acid bacteria
Production of L-lactic acid from a mixture of xylose and glucose by co-cultivation of lactic acid bacteria
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DOI：
10.1007/s00253-004-1671-x
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发表时间：
2004-12-01
影响因子：
5
通讯作者：
Tanaka, T
中科院分区：
文献类型：
--
作者：
Taniguchi, M;Tokunaga, T;Tanaka, T
本文描述了从木质纤维素水解产物木糖或木糖和葡萄糖的混合物中高产率生产光学纯乳酸的方法。在单一培养中，当使用MRS培养基时，从50 g/l木糖中产生38 g/l光学纯度为96%对映体过量（ee）的乳酸和6.4 g/l乙酸。当50 g/l木糖和100 g/l葡萄糖的混合物用作E.单独使用casseliflavus时，葡萄糖在培养的早期阶段转化为乳酸，但木糖几乎不被消耗。在共培养条件下，E.同时接种对葡萄糖特异的干酪乳杆菌和酪黄乳杆菌，在葡萄糖几乎被消耗之后很少或不产生乳酸。采用两段接种共培养法（E.在L.接种干酪细胞）导致50 g/l木糖和100 g/l葡萄糖的完全消耗。共培养192 h，乳酸的光学纯度达到96%ee，达到95 g/l。使用对每种糖特异的两种微生物的这种共培养被认为是一种有前景的培养技术，用于从源自木质纤维素的糖混合物有效生产乳酸。
The production of optically pure lactic acid in a high yield from xylose or a mixture of xylose and glucose, which is a model hydrolysate of lignocellulose, is described. In a single cultivation, Enterococcus casseliflavus produced 38 g/l of lactic acid with an optical purity of 96% enantiomeric excess (ee) and 6.4 g/l of acetic acid from 50 g/l of xylose when MRS medium was used. When a mixture of 50 g/l of xylose and 100 g/l of glucose was used as the carbon source in a cultivation of E. casseliflavus alone, glucose was converted to lactic acid in the early phase of the cultivation but xylose was hardly consumed. In a co-cultivation where E. casseliflavus and Lactobacillus casei specific for glucose were simultaneously inoculated, little or no lactic acid was produced after the glucose was almost consumed. A co-cultivation with two-stage inoculation ( in which E. casseliflavus was added at a cultivation time of 40 h after L. casei cells were inoculated) resulted in complete consumption of 50 g/l of xylose and 100 g/l of glucose. In the co-cultivation, 95 g/l of lactic acid with a high optical purity of 96% ee was obtained at 192 h. Such a co-cultivation using two microorganisms specific for each sugar is considered to be one promising cultivation technique for the efficient production of lactic acid from a sugar mixture derived from lignocellulose.