Hyperbaric Oxygen Therapy Dampens Inflammatory Cytokine Production and Does Not Worsen the Cardiac Function and Oxidative State of Diabetic Rats
Hyperbaric Oxygen Therapy Dampens Inflammatory Cytokine Production and Does Not Worsen the Cardiac Function and Oxidative State of Diabetic Rats
复制标题
DOI：
10.3390/antiox8120607
复制
发表时间：
2019-12-01
期刊：
影响因子：
7
通讯作者：
Horvath, Eszter Maria
中科院分区：
文献类型：
--
作者：
Benko, Rita;Miklos, Zsuzsanna;Horvath, Eszter Maria
高压氧治疗 (HBOT) 常用于软组织损伤后以及伤口溃烂的糖尿病患者；然而，它增加氧化应激的能力却令人怀疑。体重 300 克的雄性 Wistar 大鼠（N = 20）通过单剂量链脲佐菌素诱导糖尿病 (DM)。 10 名糖尿病患者 (DMHBOT) 和 10 名对照者 (CHBOT) 在糖尿病第 3 周后接受了 12 次长达一小时的高压氧治疗方案 (2.5 bar)。有十只动物仍未得到治疗。糖尿病诱导八周后，我们测量了24小时血糖曲线和心血管功能（超声心动图和主动脉舒张能力）。测量血浆中的丙二醛（MDA）和细胞因子水平。评估了心脏和主动脉组织中的聚（ADP-核糖）聚合酶（PARP）活性。 HBOT 并没有改变大部分心血管参数。心脏和主动脉组织中的 PARylation 使糖尿病大鼠血浆 MDA 水平升高。 HBOT 可防止糖尿病动物中 MDA 的增加。此外，促炎细胞因子诱导的中性粒细胞趋化剂-1 (CINC-1) 的水平和抗炎组织金属蛋白酶-1 抑制剂的水平在糖尿病或高氧条件下没有改变。我们的结果表明，HBOT 不会增加长期氧化应激，并且与训练类似，TBARS 产物、硝基酪氨酸形成和聚（ADP-核糖基）化可能因高氧而缓解。
Hyperbaric oxygen therapy (HBOT) is frequently used after soft tissue injuries and in diabetic patients with ulcerated wounds; however, its ability to increase oxidative stress casts doubts. Diabetes (DM) in male Wistar rats (N = 20) weighing 300 g were induced by a single dose of streptozotocin. Ten diabetics (DMHBOT) and 10 controls (CHBOT) underwent a one-hour long hyperbaric oxygen treatment protocol (2.5 bar) 12 times after the 3rd week of diabetes. Ten animals remained untreated. Eight weeks after diabetes induction, we measured the 24-hour blood glucose profile and cardiovascular function (sonocardiography and the relaxation ability of aortae). Malonyl-dialdehyde (MDA) and cytokine levels were measured in blood plasma. Poly(ADP-ribose) polymerase (PARP) activity was estimated in cardiac and aortic tissue. HBOT did not alter most of the cardiovascular parameters. PARylation in cardiac and aortic tissues, plasma MDA levels were elevated in diabetic rats. HBOT prevented the increase of MDA in diabetic animals. In addition, levels of the pro-inflammatory cytokine-induced neutrophil chemoattractant-1 (CINC-1) the levels of anti-inflammatory tissue inhibitor of metalloproteases-1 were not altered in diabetes or in hyperoxia. Our results suggest that HBOT does not increase long-term oxidative stress, and, similar to training, the TBARS products, nitrotyrosine formation and poly(ADP-ribosyl)ation may be eased as a result of hyperoxia.