United States Standard Diphtheria Toxin for the Schick Text and the Erythema Potency Assay for the Schick Text Dose
United States Standard Diphtheria Toxin for the Schick Text and the Erythema Potency Assay for the Schick Text Dose
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Schick Text 的美国标准白喉毒素和 Schick Text 剂量的红斑效力测定
作者：
M. Barile;R. W. Kolb;M. Pittman
一种白喉毒素显示出高毒性和亲和性，并以多种比例与抗毒素结合，被选为美国标准白喉毒素进行希克试验并冷冻干燥。每瓶的测定值为1.09 Lf, 1.09 Lr， 1090 Schick试验剂量（STD）， 33 LD80, 38 LD50和43,000最小皮肤反应剂量。1个STD （Lr/1000）比1个国际标准（Lf/ 1000）的毒性略高。实验表明，用豚鼠红斑毒性法测定希克试验毒素的效价，相对于标准的毒性，具有高度的可重复性，并且比致死（最小致死剂量）试验的可重复性显著提高，定义为1 Lr/1000的STD相当于大约1/50的最小致死剂量。红斑效力测定法在1969年生效的美国希克试验毒素标准中被规定。
A diphtheria toxin shown to have high toxicity and avidity and to combine with antitoxin in multiple proportions was selected for the U.S. standard diphtheria toxin for the Schick test and freeze-dried. Assayed values per vial were 1.09 Lf, 1.09 Lr, 1,090 Schick test doses (STD), 33 LD80, 38 LD50, and 43,000 minimum skin reactive doses. One STD (Lr/1000) is slightly more toxic than one unit of the international standard (Lf/1,000). Experiments showed that the potency assay of Schick test toxin by guinea pig erythema toxicity, determined relative to the toxicity of the standard, was highly reproducible and significantly more reproducible than the lethal (minimum lethal dose) test and that the STD, defined as one Lr/1000, was equivalent to approximately 1/50 minimum lethal dose. The erythema potency assay was prescribed in the U.S. standards for Schick test toxin effective in 1969.