Prethymic T-cell development defined by the expression of paired immunoglobulin-like receptors
Prethymic T-cell development defined by the expression of paired immunoglobulin-like receptors
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DOI：
10.1038/sj.emboj.7600878
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发表时间：
2005-12-07
期刊：
影响因子：
11.4
通讯作者：
Kawamoto, H
中科院分区：
文献类型：
--
作者：
Masuda, K;Kubagawa, H;Kawamoto, H
T细胞在胸腺中由胸腺外来源的祖细胞产生。由于没有特异性标记，胸腺定植前祖细胞的发育途径仍不清楚。在这里，我们表明，小鼠胎肝和血液中的祖细胞，这是能够产生T细胞，NK细胞和树突状细胞，但不是B细胞，可以通过其配对的免疫球蛋白样受体（PIR）的表面表达分离。PIR表达维持到最早的胸腺内阶段，然后在CD25表达开始之前下调。与胸腺内祖细胞不同，胸腺前PIR+祖细胞的产生不需要Hes1介导的Notch信号传导。这些发现揭示了T细胞发育的胸腺前阶段，该阶段被编程为胸腺的移民，其在遗传上可与胸腺内阶段分离。
T cells are produced in the thymus from progenitors of extrathymic origin. As no specific markers are available, the developmental pathway of progenitors preceding thymic colonization remains unclear. Here we show that progenitors in murine fetal liver and blood, which are capable of giving rise to T cells, NK cells and dendritic cells, but not B cells, can be isolated by their surface expression of paired immunoglobulin-like receptors (PIR). PIR expression is maintained until the earliest intrathymic stage, then downregulated before the onset of CD25 expression. Unlike intrathymic progenitors, generation of prethymic PIR+ progenitors does not require Hes1-mediated Notch signaling. These findings disclose a prethymic stage of T-cell development programmed for immigration of the thymus, which is genetically separable from intrathymic stages.