ANTIFUNGAL EFFECTS OF PEROXIDASE SYSTEMS
ANTIFUNGAL EFFECTS OF PEROXIDASE SYSTEMS
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DOI：
10.1128/jb.99.2.361-365.1969
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发表时间：
1969-01-01
影响因子：
3.2
通讯作者：
LEHRER, RI
中科院分区：
文献类型：
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作者：
LEHRER, RI
在过氧化氢和碘化钾、氯化钠或溴化钾存在下，纯化的人髓过氧化物酶对几种念珠菌迅速致死。其杀念珠菌活性被氰化物、氟化物和叠氮化物以及酶的热灭活所抑制。由d-氨基酸氧化酶、黄嘌呤-腺嘌呤二核苷酸和d-丙氨酸组成的过氧化氢产生系统可以代替过氧化氢用于杀念珠菌系统。辣根过氧化物酶和人嗜酸性粒细胞颗粒也发挥杀念珠菌活性的碘化物和过氧化氢的存在下，然而，不像髓过氧化物酶或中性粒细胞颗粒，这些过氧化物酶来源是无活性的氯化物取代碘化物。酵母菌属、地丝菌属和红酵母属种的细胞以及烟曲霉和A.黑猩猩也被髓过氧化物酶、碘化物和过氧化氢的组合杀死。过氧化物酶与过氧化氢生成系统功能相关，可以为吞噬细胞提供杀死许多真菌物种的能力。
In the presence of hydrogen peroxide and either potassium iodide, sodium chloride, or potassium bromide, purified human myeloperoxidase was rapidly lethal to several species ofCandida. Its candidacidal activity was inhibited by cyanide, fluoride, and azide, and by heat inactivation of the enzyme. A hydrogen peroxidegenerating system consisting ofd-amino acid oxidase, flavine-adenine dinucleotide, andd-alanine could replace hydrogen peroxide in the candidacidal system. Horseradish peroxidase and human eosinophil granules also exerted candidacidal activity in the presence of iodide and hydrogen peroxide; however, unlike myeloperoxidase or neutrophil granules, these peroxidase sources were inactive when chloride replaced iodide. Cells ofSaccharomyces, Geotrichum, andRhodotorulaspecies, and spores ofAspergillus fumigatusandA. nigerwere also killed by the combination of myeloperoxidase, iodide, and hydrogen peroxide. Peroxidases, functionally linked to hydrogen peroxide-generating systems, could provide phagocytic cells with the ability to kill many fungal species.