Synthesis and properties of DNA-PNA chimeric oligomers
Synthesis and properties of DNA-PNA chimeric oligomers
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DOI：
10.1093/nar/24.17.3357
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发表时间：
1996-09-01
影响因子：
14.9
通讯作者：
Brown, T
中科院分区：
文献类型：
--
作者：
Finn, PJ;Gibson, NJ;Brown, T
以3-氨基-1，2-丙二醇为起始原料制备了腺嘌呤、胸腺嘧啶和胞嘧啶RNA单体。用苯甲酰基保护A和C PNA单体杂环碱基的环外胺，用单甲氧基三苯甲基保护主链伯胺，胸腺嘧啶和胞嘧啶PNA单体与标准DNA合成单体结合使用以产生嵌合PNA DNA（PDC）寡聚体。紫外线熔解研究证实，这些寡聚体与互补DNA链形成稳定的杂交体，并且DNA中的错配，但RNA部分中的错配更是导致双链体不稳定。
Adenine, thymine and cytosine RNA monomers have been prepared using 3-amino-1,2-propanediol as a starting material, The benzoyl group was used to protect the exocyclic amines of the heterocyclic bases of A and C PNA monomers and the backbone primary amine was protected with the monomethoxytrityl group, The thymine and cytosine PNA monomers were used in conjunction with standard DNA synthesis monomers to produce chimeric PNA DNA (PDC) oligomers. Ultraviolet melting studies confirmed that these oligomers form stable hybrids with complementary DNA strands and that mismatches in the DNA but more so in the RNA sections lead to duplex destabilisation.