Breeding new strains of tilapia: development of an artificial center of origin and linkage map based on AFLP and microsatellite loci
Breeding new strains of tilapia: development of an artificial center of origin and linkage map based on AFLP and microsatellite loci
复制标题
DOI：
10.1016/s0044-8486(99)00335-x
复制
发表时间：
2000-05-02
期刊：
影响因子：
4.5
通讯作者：
May, B
中科院分区：
文献类型：
--
作者：
Agresti, JJ;Seki, S;May, B
基于植物育种的思想和分子生物学提供的机会，1995年启动了一项计划，从一种合成种群（人工起源中心，AGO）中获得遗传优越的罗非鱼，该种群是由鱼的染色群杂交产生的：Oreochromis niloticus[野生型（on）和红色（ROn）菌株]，O. aureus (Oa)， O, mossambicus （Om）和Sarotherodon galilaeus （Sg）。三向交叉科（3WC）和四向交叉科（4WC）已经产生，因此这四个物种在AGO中都有代表。利用微卫星和AFLP（扩增片段长度多态性）DNA标记建立了Om X （Oa X ROn）家族亲本的基因组图谱。母亲本共有78个分离标记（微卫星17个，aflp 61个）。其中，61个（13个微卫星，49个aflp）连接在14个连锁群中，共覆盖514厘米（cM）。第一代（F-1）杂交父本共有229个分离标记（62个微卫星，167个AFLPS），其中214个（60个微卫星，154个AFLPS）被连锁在24个连锁群中，总面积达1632 cM。这些图谱的构建是罗非鱼耐冷、耐盐和胴体品质数量性状位点（QTL）分子标记辅助育种计划的关键一步。(C) 2000 Elsevier Science B.V.版权所有
Based on ideas from plant breeding and the opportunities offered by molecular biology, a program was initiated in 1995 to derive genetically superior tilapia from a synthetic stock (artificial center of origin, AGO) produced by inter-crossing dye groups of fish: Oreochromis niloticus [wild-type (On) and red (ROn) strains], O. aureus (Oa), O, mossambicus (Om), and Sarotherodon galilaeus (Sg). Three-way cross families (3WC) and four-way cross families (4WC) have been produced, so that all four species are represented in the AGO. A genomic map has been created for each of the parents in an Om X (Oa X ROn) family using microsatellite and AFLP (amplified fragment length polymorphism) DNA markers. nle female (Om) parent had a total of 78 segregating markers (17 microsntellites, 61 AFLPs). Of these, 61 (13 microsatellites, 49 AFLPs) were linked in 14 linkage groups covering a total of 514 centimorgans (cM). The first generation (F-1) hybrid male parent had a total of 229 segregating markers (62 microsatellites, 167 AFLPS), of which 214 (60 microsatellites, 154 AFLPs) were linked in 24 linkage groups covering a total of 1632 cM. The construction of these maps is a key Step in a molecular marker-assisted breeding program to detect quantitative trait loci (QTL) for cold and salinity tolerance and carcass quality in tilapia. (C) 2000 Elsevier Science B.V. All rights reserved.