Myogenic induction of aligned mesenchymal stem cell sheets by culture on thermally responsive electrospun nanofibers
Myogenic induction of aligned mesenchymal stem cell sheets by culture on thermally responsive electrospun nanofibers
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DOI：
10.1002/adma.200602159
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发表时间：
2007-10-05
影响因子：
29.4
通讯作者：
Leong, Kam W.
中科院分区：
文献类型：
--
作者：
Dang, Jiyoung M.;Leong, Kam W.
具有排列的纳米纤维的形貌活性表面的热可逆培养基质能够诱导细胞骨架排列和细胞核伸长。这些形态学变化诱导干细胞的肌源性分化。分化的细胞可以通过基质的热诱导溶解以片形式回收。能够提供地形线索以产生经活化以分化成特定谱系的对齐的干细胞片层的表面可对用于再生医学应用的组织构建体的工程化具有显著影响。
A thermally reversible culture substrate with a topographically active surface of aligned nanofibers is able to induce cytoskeletal alignment and nucleus elongation. These morphological changes induce myogenic differentiation in stem cells. The differentiated cells can be recovered in sheet form by thermally induced dissolution of the substrate. A surface able to provide topographical cues to create an aligned stem cell sheet, activated to differentiate to a specific lineage, can have a significant impact on engineering of tissue constructs for regenerative medicine applications.