IMMUNOCYTOCHEMICAL STUDY OF PEPTIDERGIC STRUCTURES IN BRUNNER GLANDS
IMMUNOCYTOCHEMICAL STUDY OF PEPTIDERGIC STRUCTURES IN BRUNNER GLANDS
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DOI：
10.1016/0016-5085(89)90380-6
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发表时间：
1989-12-01
期刊：
影响因子：
29.4
通讯作者：
CHAYVIALLE, JA
中科院分区：
文献类型：
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作者：
BOSSHARD, A;CHERYCROZE, S;CHAYVIALLE, JA
通过免疫荧光研究了人类布伦纳腺的神经和内分泌肽能结构。内分泌细胞储存的免疫反应成分分别类似于生长抑素14、生长抑素28的氨基末端部分(1-14)、胃泌素胆囊收缩素和肽YY，分布在整个腺泡中。肽能神经结构含有与血管活性肠肽、肽组氨酸蛋氨酸、P物质、神经肽Y和胃泌素释放肽免疫学相关的物质。后一种肽在进入腺泡的离散纤维中检测到，但在粘膜下层的细胞体内没有检测到。所有其他神经肽都储存在纤维中，分离或聚集成束，并储存在靠近腺泡的粘膜下神经节的核周中。使用针对胰多肽、促胰液素、胃动素、神经降压素或降钙素基因相关肽的抗血清未检测到免疫反应结构。结果表明，几种调节肽可能参与人类布伦纳氏腺的控制。
Nervous and endocrine peptidergic structures in human Brunner''s glands were studied by immunofluorescence. Endocrine cells storing immunoreactive components respectively similar to somatostatin 14, the amino-terminal portion (1-14) of somatostatin 28, gastrin-cholecystokinin, and peptide YY were distributed throughout the acini. Peptidergic nerve structures contained materials immunologically related to vasoactive intestinal peptide, peptide histidine methionine, substance P, neuropeptide Y, and gastrin-releasing peptide. The latter peptide was detected in discrete fibers running into the acini but within no cell body in the submucosa. All other neuropeptides were stored in fibers, isolated or grouped in bundles, and in perikarya of submucosal ganglia close to the acini. No immunoreactive structures were detected using antisera directed against pancreatic polypeptide, secretin, motilin, neurotensin, or calcitonin gene-related peptide. The results suggest that several regulatory peptides may be involved in the control of Brunner''s glands in humans.