Immunogenicity of outer membrane protein of Pasteurella multocida in buffalo calves
Immunogenicity of outer membrane protein of Pasteurella multocida in buffalo calves
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DOI：
10.1016/s0378-1135(96)00066-1
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发表时间：
1996-10-01
影响因子：
3.3
通讯作者：
More, T
中科院分区：
文献类型：
--
作者：
Pati, US;Srivastava, SK;More, T
采用SDS-PAGE技术对多杀性巴氏杆菌血清型B:2的外膜蛋白（OMP）进行了提取和鉴定。观察到分子量为88 ~ 25 kDa的10条主要多肽带。免疫印迹分析表明，m44,37和30kda的多肽是主要的免疫原。接种了OMP疫苗或商业出血性败血症油佐剂疫苗的水牛犊牛在接种后第21天（pv）的平均对数（10）ELISA滴度最高。使用间接血凝试验在这些动物中检测到的抗体滴度低于ELISA滴度，但抗体反应的模式相似。小鼠被动保护实验显示，在第21天和第26天收集的两组血清对攻击感染具有最大的保护作用。在毒力强的多杀性假单胞菌细胞攻击后，所有5只接种了OMP的动物都存活了下来，而接种了HS油佐剂的3只动物中只有2只经受住了攻击。结果表明，OMP具有保护作用，可用于出血性败血症疫苗。
Outer membrane protein (OMP) from Pasteurella multocida serotype B:2 was extracted and characterized using SDS-PAGE. Ten major polypeptide bands of MW 88 to 25 kDa were observed. Immunoblotting suggested that the polypeptides of MW 44, 37 and 30 kDa were the major immunogens. Buffalo calves vaccinated with the OMP vaccine or a commercial haemorrhagic septicaemia oil adjuvant vaccine developed highest mean log(10) ELISA titres day 21 postvaccination (pv). Antibody titres detectable in these animals using an indirect haemagglutination assay were lower than the ELISA titres but the pattern of the antibody response was similar. A passive mouse protection assay revealed that the maximum protection against the challenge infection was conferred by the serum collected from both the groups on day 21 pv and 26 pv. Following challenge with virulent P. multocida cells, all the five OMP vaccinated animals survived whereas only 2 out of the 3 HS oil adjuvant vaccinated animals withstood the challenge. Results suggested that OMP was protective and could be used in vaccines against haemorrhagic septicaemia.