New perspective to improve dentin–adhesive interface stability by using dimethyl sulfoxide wet-bonding and epigallocatechin-3-gallate
New perspective to improve dentin–adhesive interface stability by using dimethyl sulfoxide wet-bonding and epigallocatechin-3-gallate
复制标题
使用二甲基亚砜湿法粘合和表没食子儿茶素-3-没食子酸酯提高牙本质-粘合界面稳定性的新视角
DOI：
10.1016/j.dental.2020.08.009
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发表时间：
2020
期刊：
影响因子：
5
通讯作者：
Huang Cui
中科院分区：
文献类型：
--
作者：
Zhang Zhongni;Yu Jian;Yao Chenmin;Yang Hongye;Huang Cui
目的探讨二甲基亚砜（DMSO）湿粘和表没食子儿茶素没食子酸酯（EGCG）对牙本质-粘接剂界面稳定性的影响。第3-5组、0.01、0.1和1重量%掺入EGCG的50%（v/v）DMSO湿粘合（0.01%、0.1%和1%EGCG/DWB）。将Single-bond通用粘合剂应用于预处理的牙本质表面，并构建复合构建物。分别在24 h水储存或1个月胶原酶老化后检测微拉伸粘结强度（μTBS）和界面纳米渗漏。原位酶谱和变形链球菌（S.结果胶原酶老化后，第4组（0.1%EGCG/DWB）和第5组（1%EGCG/DWB）的μTBS无明显下降（p> 0.05），且高于其他3组（p< 0.05）。无论胶原酶老化与否，第4组和第5组的纳米渗漏表达均低于其他三组（p< 0.05）。第4组和第5组的混合层内的金属蛋白酶活性受到抑制。1%EGCG/DWB预处理（第5组）可有效抑制S。本研究提示DMSO湿粘接剂和EGCG的协同作用能有效提高牙本质-粘接剂界面的稳定性。这种策略为临床医生提供了有前途的好处，以实现理想的牙本质粘结性能，并防止继发性龋，从而延长粘接剂的寿命。
ObjectivesTo determine whether dentin–adhesive interface stability would be improved by dimethyl sulfoxide (DMSO) wet-bonding and epigallocatechin-3-gallate (EGCG).MethodsEtched dentin surfaces from sound third molars were randomly assigned to five groups according to different pretreatments: group 1, water wet-bonding (WWB); group 2, 50% (v/v) DMSO wet-bonding (DWB); groups 3–5, 0.01, 0.1, and 1 wt% EGCG-incorporated 50% (v/v) DMSO wet-bonding (0.01%, 0.1%, and 1%EGCG/DWB). Singlebond universal adhesive was applied to the pretreated dentin surfaces, and composite buildups were constructed. Microtensile bond strength (μTBS) and interfacial nanoleakage were respectively examined after 24 h water storage or 1-month collagenase ageing. In situ zymography andStreptococcus mutans(S. mutans) biofilm formation were also investigated.ResultsAfter collagenase ageing, μTBS of groups 4 (0.1%EGCG/DWB) and 5 (1%EGCG/DWB) did not decrease (p> 0.05) and was higher than that of the other three groups (p< 0.05). Nanoleakage expression of groups 4 and 5 was less than that of the other three groups (p< 0.05), regardless of collagenase ageing. Metalloproteinase activities within the hybrid layer in groups 4 and 5 were suppressed. Furthermore, pretreatment with 1%EGCG/DWB (group 5) efficiently inhibitedS. mutansbiofilm formation along the dentin–adhesive interface.SignificanceThis study suggested that the synergistic action of DMSO wet-bonding and EGCG can effectively improve dentin–adhesive interface stability. This strategy provides clinicians with promising benefits to achieve desirable dentin bonding performance and to prevent secondary caries, thereby extending the longevity of adhesive restorations.