Inhibitory effect of epigallocatechin gallate on adhesion of murine melanoma cells to laminin
Inhibitory effect of epigallocatechin gallate on adhesion of murine melanoma cells to laminin
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DOI：
10.1016/s0304-3835(01)00685-1
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发表时间：
2001-11-08
期刊：
影响因子：
9.7
通讯作者：
Isemura, M
中科院分区：
文献类型：
--
作者：
Suzuki, Y;Isemura, M
我们研究了五种绿茶儿茶素对小鼠黑色素瘤B16细胞粘附层粘连蛋白的影响。（-）-表儿茶素没食子酸酯（EGCG）和(-)-表儿茶素没食子酸酯在培养基中抑制细胞粘附。经EGCG预处理后，其与层粘连蛋白的粘附力也受到损害。亲和层析显示了层粘连蛋白与EGCG的结合亲和性。这些数据表明，EGCG对黑色素瘤细胞粘附层粘连蛋白的抑制作用包括在先前报道的EGCG和绿茶输注引起的转移抑制机制中。(C) 2001爱思唯尔科学爱尔兰有限公司版权所有。
We examined the effects of five kinds of green tea catechin on the adhesion of mouse melanoma B16 cells to laminin. (-)-Epigallocatechin gallate (EGCG) and (-)-epicatechin gallate in the culture medium were found to inhibit the cell adhesion. The adhesion to laminin pre-treated with EGCG was also impaired. Affinity chromatography revealed the binding affinity between laminin and EGCG. These data suggest that the inhibitory effect of EGCG on adhesion of melanoma cells to laminin is included in the mechanism(s) of previously reported metastasis inhibition elicited by EGCG and green tea infusion. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.