DNA Methylation Profiles in Placenta and Its Association with Gestational Diabetes Mellitus
DNA Methylation Profiles in Placenta and Its Association with Gestational Diabetes Mellitus
复制标题
胎盘 DNA 甲基化谱及其与妊娠期糖尿病的关系
DOI：
10.1055/s-0034-1398666
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发表时间：
2015-05-01
影响因子：
1.8
通讯作者：
Hu, Y.
中科院分区：
文献类型：
--
作者：
Rong, C.;Cui, X.;Hu, Y.
Emerging evidences indicate that placenta plays a critical role in gestational diabetes mellitus (GDM). DNA methylation could be associated with altered placental development and functions. This study is to uncover the genome-wide DNA methylation patterns in this disorder. DNA methylation was measured at >385000 CpG sites using methylated DNA immunoprecipitation (MeDIP) and a huamn CpG island plus promoter microarray. We totally identified 6641 differentially methylated regions (DMRs) targeting 3320 genes, of which 2729 DMRs targeting 1399 genes, showed significant hypermethylation in GDM relative to the controls, whereas 3912 DMRs targeting 1970 genes showed significant hypomethylation. Functional analysis divided these genes into different functional networks, which mainly involved in the pathways of cell growth and death regulation, immune and inflammatory response and nervous system development. In addition, the methylation profiles and expressions of 4 loci (RBP4, GLUT3, Resistin and PPAR) were validated by BSP for their higher log2 ratio and potential functions with energy metabolism. This study demonstrates aberrant patterns of DNA methylation in GDM which may be involved in the pathophysiology of GDM and reflect the fetal development. Future work will assess the potential prognostic and therapeutic value for these findings in GDM.
