Transforming growth factor-beta modulates plasminogen activator activity and plasminogen activator inhibitor type-1 expression in human keratinocytes in vitro.
Transforming growth factor-beta modulates plasminogen activator activity and plasminogen activator inhibitor type-1 expression in human keratinocytes in vitro.
复制标题
转化生长因子-β 在体外调节人角质形成细胞中纤溶酶原激活剂活性和纤溶酶原激活剂抑制剂 1 型表达。
DOI：
10.1111/1523-1747.ep12505603
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Clark,RA
中科院分区：
文献类型：
--
作者：
Wikner,NE;Elder,JT;Persichitte,KA;Mink,P;Clark,RA
转化生长因子β (TGF-β)是一种影响细胞生长、分化和细胞外基质（ECM）代谢的多功能介质。由于TGF-β刺激培养的人角质形成细胞中纤维连接蛋白的表达，我们希望确定它是否也可能通过纤溶酶原激活剂(PA) -纤溶酶原激活剂抑制剂（PAI）系统影响ECM的降解。人角化细胞在TGF-β存在下培养时，用1型PAI单特异性抗血清（PAT-1）免疫荧光显示细胞和ECM染色增强。抗血清还在条件培养基中鉴定出Mr 50,000蛋白，TGF-β显著增强该蛋白。定量RNA印迹分析显示PAL-1 mRNA受到相应的刺激。TGF-β显著降低条件培养基总纤溶酶原激活活性。酶谱图显示，这至少部分是由于分泌的尿激酶活性降低。TGF-β可能在伤口愈合过程中稳定角质形成细胞合成的临时基质中发挥重要作用。
Transforming growth factor beta (TGF-β) is a multifunctional mediator with effects on cellular growth, differentiation, and extracellular matrix (ECM) metabolism. Because TGF-β stimulates fibronectin expression in cultured human keratinocytes, we wished to determine whether it might also affect ECM degradation through the plasminogen activator (PA) – plasminogen activator inhibitor (PAI) system. Immunofluorescence of human keratinocytes using a monospecific antiserum to type 1 PAI (PAT-1) showed enhanced cellular and ECM staining when they were cultured in the presence of TGF-β. The antiserum also identified an Mr 50,000 protein in conditioned media that was markedly enhanced by TGF-β. A corresponding stimulation of PAL-1 mRNA was demonstrated by quantitative RNA blot analysis. Total plasminogen activating activity of conditioned medium was markedly decreased by TGF-β. Zymography showed this to be at least partially due to decreased secreted urokinase activity. TGF-β may play an important role in stabilizing the provisional matrix synthesized by keratinocytes in healing wounds.