DEVELOPMENTALLY CONTROLLED EXPRESSION OF A GENE ASSOCIATED WITH SELF-INCOMPATIBILITY IN NICOTIANA-ALATA
DEVELOPMENTALLY CONTROLLED EXPRESSION OF A GENE ASSOCIATED WITH SELF-INCOMPATIBILITY IN NICOTIANA-ALATA
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DOI：
10.1038/326099a0
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发表时间：
1987-03-05
期刊：
影响因子：
64.8
通讯作者：
CLARKE, AE
中科院分区：
文献类型：
--
作者：
CORNISH, EC;PETTITT, JM;CLARKE, AE
在许多开花植物物种中，自交不亲和系统通常由具有多个等位基因的单个基因（S基因）1控制，使个体植物能够识别并拒绝自己的花粉。在配子体决定的系统中，自花花粉管的生长在花柱中或偶尔在子房中被阻止。这种自交不亲和性的表达是发育调节的，在成熟的花朵中最强，在未成熟的花柱中较弱1，2。我们用一个互补的DNA克隆，相信它编码烟草alata2，3的S2等位基因，通过原位杂交检测S2信使RNA。我们在这里报告，该基因的表达发生在柱头和整个分泌组织，而不是在其他部分，成熟雌蕊。在未成熟的花中，S2 mRNA仅限于柱头的增殖表皮。S2基因的表达与不亲和性的表达有很好的相关性。
In many flowering plant species, a system of self-incompatibility, typically controlled by a single gene with multiple alleles (S-gene)1, enables individual plants to recognize and reject their own pollen. In gametophytically determined systems, the growth of self-pollen tubes is arrested in the style or occasionally the ovary. The expression of this self-incompatibility is developmentally regulated, being strongest in mature flowers and weak in immature styles1,2. We have used a complementary DNA clone, believed to encode theS2-allele ofNicotiana alata2,3, to detectS2messenger RNA byin situhybridization to sections. We report here that expression of the gene occurs in the stigma and throughout the secretory tissue, but not in other parts, of mature pistils. In immature flowers,S2mRNA is confined to the proliferated epidermis of the stigma. We conclude thatS2-gene expression correlates well with the expression of incompatibility.