The pH-Responsive Transcription Factors YlRim101 and Mhy1 Regulate Alkaline pH-Induced Filamentation in the Dimorphic Yeast Yarrowia lipolytica.
The pH-Responsive Transcription Factors YlRim101 and Mhy1 Regulate Alkaline pH-Induced Filamentation in the Dimorphic Yeast Yarrowia lipolytica.
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pH 响应转录因子 YlRim101 和 Mhy1 调节二态性酵母解脂耶氏酵母中碱性 pH 诱导的丝状形成
DOI：
10.1128/msphere.00179-21
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发表时间：
2021-05-19
期刊：
影响因子：
4.8
通讯作者：
Gao XD
中科院分区：
文献类型：
--
作者：
Shu T;He XY;Chen JW;Mao YS;Gao XD
环境pH影响细胞生长和分化。在解脂耶氏酵母中，中性-碱性pH强烈诱导酵母到丝状体的转变。然而，控制碱性pH诱导的抑制的调节机制一直不清楚。在这里，我们表明，pH响应转录因子Y。解脂酵母Rim 101（YlRim 101）是碱诱导表达的主要调节因子，因为YlRim 101的缺失严重损害了碱性pH下的表达，而组成型活性的YlRim 1011 -330突变体在酸性pH下温和地诱导表达。其中之一，细胞表面糖苷酶基因YlPHR 1，在生长，细胞壁功能，并在碱性pH值下的抑制中起着关键作用。这一发现表明，YlRim 101通过控制这些基因的表达促进在碱性pH值下的抑制。我们还表明，除了YlRim 101，Msn 2/Msn 4样转录因子Mhy 1是高度上调在碱性pH值和是必不可少的。然而，不像YlRim 101，它专门调节碱诱导的抑制，MHY 1调节碱和葡萄糖诱导的抑制，因为MHY 1的缺失废除了他们两个，而MHY 1的过表达诱导强抑制，无论pH值或葡萄糖的存在。最后，我们发现，YlRim 101和Mhy 1积极共调节7细胞壁蛋白基因在碱性pH值，包括YlPHR 1和5个细胞表面粘附素样基因，其中三个似乎促进recrystalization。总之，这些结果揭示了YlRim 101的保守作用和Mhy 1在Y.解脂重要性除了白色念珠菌之外，在二态真菌中，控制pH调节的成丝的调节机制尚不清楚。在这里，我们研究了在解脂耶氏酵母（Yarrowia lipolytica）中碱性pH诱导的发酵的调节。白色念珠菌。我们的研究结果表明，转录因子YlRim 101和Msn 2/Msn 4样转录因子Mhy 1的主要监管机构，促进在碱性pH值的表达。他们控制的细胞壁组织和表达的细胞壁蛋白基因的数量是重要的。我们的研究结果表明，Rim 101/PacC同源物在二型真菌中的pH调节表达中起着保守的作用。
Environmental pH influences cell growth and differentiation. In the dimorphic yeast Yarrowia lipolytica, neutral-alkaline pH strongly induces the yeast-to-filament transition. However, the regulatory mechanism that governs alkaline pH-induced filamentation has been unclear. Here, we show that the pH-responsive transcription factor Y. lipolytica Rim101 (YlRim101) is a major regulator of alkaline-induced filamentation, since the deletion of YlRIM101 severely impaired filamentation at alkaline pH, whereas the constitutively active YlRIM1011-330 mutant mildly induced filamentation at acidic pH. YlRim101 controls the expression of the majority of alkaline-regulated cell wall protein genes. One of these, the cell surface glycosidase gene YlPHR1, plays a critical role in growth, cell wall function, and filamentation at alkaline pH. This finding suggests that YlRim101 promotes filamentation at alkaline pH via controlling the expression of these genes. We also show that, in addition to YlRim101, the Msn2/Msn4-like transcription factor Mhy1 is highly upregulated at alkaline pH and is essential for filamentation. However, unlike YlRim101, which specifically regulates alkaline-induced filamentation, Mhy1 regulates both alkaline- and glucose-induced filamentation, since the deletion of MHY1 abolished them both, whereas the overexpression of MHY1 induced strong filamentation irrespective of the pH or the presence of glucose. Finally, we show that YlRim101 and Mhy1 positively coregulate seven cell wall protein genes at alkaline pH, including YlPHR1 and five cell surface adhesin-like genes, three of which appear to promote filamentation. Together, these results reveal a conserved role of YlRim101 and a novel role of Mhy1 in the regulation of alkaline-induced filamentation in Y. lipolytica. IMPORTANCE The regulatory mechanism that governs pH-regulated filamentation is not clear in dimorphic fungi except in Candida albicans. Here, we investigated the regulation of alkaline pH-induced filamentation in Yarrowia lipolytica, a dimorphic yeast distantly related to C. albicans. Our results show that the transcription factor YlRim101 and the Msn2/Msn4-like transcription factor Mhy1 are the major regulators that promote filamentation at alkaline pH. They control the expression of a number of cell wall protein genes important for cell wall organization and filamentation. Our results suggest that the Rim101/PacC homologs play a conserved role in pH-regulated filamentation in dimorphic fungi.