Limb chondrogenesis is compromised in the versican deficient hdf mouse.
Limb chondrogenesis is compromised in the versican deficient hdf mouse.
复制标题
DOI：
10.1016/j.bbrc.2005.06.189
复制
发表时间：
2005-09
影响因子：
3.1
通讯作者：
Dennis R. Williams;Ashley R. Presar;A. Richmond;C. Mjaatvedt;S. Hoffman;A. A. Capehart-A.
中科院分区：
文献类型：
--
作者：
Dennis R. Williams;Ashley R. Presar;A. Richmond;C. Mjaatvedt;S. Hoffman;A. A. Capehart-A.
有人建议，基质蛋白聚糖，多功能蛋白聚糖，可能在肢体骨骼发育的早期事件中发挥功能性作用，主要是凭借其在前软骨间充质聚集的时空表达模式。多功能蛋白聚糖缺陷的hdf转基因小鼠提供了第一个模型来探索无效的成熟多功能蛋白聚糖对肢体软骨形成的影响。由于hdf纯合子胚胎在肢体软骨分化前的致死性，采用高密度微团培养来比较hdf突变体与野生型肢体间充质的软骨形成能力。在纯合子hdf间充质中，聚集严重受损，在6天的培养期间既没有检测到软骨特征性II型胶原，也没有检测到阿新蓝阳性病灶。然而，hdf突变间充质的三维培养表明，在允许的环境中，突变细胞也表达II型胶原。结果强烈表明，成熟的多功能蛋白聚糖是必不可少的前软骨聚集和随后的软骨分化。
It has been suggested that the matrix proteoglycan, versican, may perform a functional role during early events of limb skeletogenesis largely by virtue of its spatiotemporal expression pattern in precartilage mesenchymal aggregations. The versican-deficient hdf transgenic mouse has provided the first model to explore the implications of a null mature versican on limb chondrogenesis. Due to lethality of hdf homozygous embryos prior to limb cartilage differentiation, high-density micromass cultures were employed to compare the chondrogenic capacity of hdf mutant limb mesenchyme to that of wild-type. In homozygous hdf mesenchyme, aggregation was severely compromised and neither cartilage-characteristic Type II collagen nor alcian blue positive foci were detected during a 6-day period of culture. Three-dimensional culture of hdf mutant mesenchyme, however, showed that in a permissive environment mutant cells also expressed Type II collagen. Results strongly suggest that mature versican proteoglycan is essential for precartilage aggregation and subsequent cartilage differentiation.