Purification and chemical characterization of α‐fetoprotein from rat and mouse
Purification and chemical characterization of α‐fetoprotein from rat and mouse
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大鼠和小鼠甲胎蛋白的纯化和化学表征
DOI：
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发表时间：
1974
期刊：
影响因子：
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通讯作者：
H. Watabe
中科院分区：
文献类型：
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作者：
H. Watabe
对大鼠的甲胎蛋白进行免疫化学纯化和表征。小鼠甲胎蛋白与大鼠甲胎蛋白抗体发生交叉反应，也被纯化。大鼠甲胎蛋白在圆盘电泳和等电聚焦中呈异质性，并且在 pH 4.76 和 5.05 处具有两个不同的等电点。类似分析时，小鼠甲胎蛋白是均质的。在十二烷基硫酸钠存在下通过凝胶电泳测定大鼠和小鼠甲胎蛋白的分子量均为 70,000。这两个样本在其他一些理化、化学和免疫学特性方面也显示出密切的相似性。
Alpha‐fetoprotein of the rat was immunochemically purified and characterized. Mouse α‐fetoprotein, which cross‐reacts with antibodies to rat α‐fetoprotein, was also purified. Rat α‐fetoprotein was heterogeneous in disc electrophoresis and isoelectric focusing and had two different isoelectric points at pH 4.76 and 5.05. Mouse α‐fetoprotein was homogeneous when analyzed similarly. The molecular weight of both rat and mouse α‐fetoprotein was 70,000 as determined by gel electrophoresis in the presence of sodium dodecyl sulfate. The two specimens revealed a close similarity also in some other physicochemical, chemical and immunological properties.