Low-power compact continuous-wave stimulated emission depletion microscopy
Low-power compact continuous-wave stimulated emission depletion microscopy
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DOI：
10.1002/jbio.202200233
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发表时间：
2022-09-13
影响因子：
2.8
通讯作者：
Qu，Junle
中科院分区：
文献类型：
--
作者：
Han，HongYi;Wang，Luwei;Qu，Junle
受激发射耗尽（STED）显微镜可以打破光学衍射障碍，并提供亚衍射分辨率。根据STED超分辨成像原理，STED的分辨率与耗尽激光的功率成正比。然而，高激光功率在很大程度上限制了对活细胞或活体的研究。此外，传统脉冲STED显微镜的高复杂性和高成本限制了该技术的应用。因此，本文描述了一种在45 × 60 cm实验板上构建的简单连续波STED（CW‐STED）系统，并结合数字增强（DE）技术，实现了低功耗超分辨率成像，具有降低系统复杂度和成本的优点。CW-STED的低系统复杂性、低成本和低功耗超分辨率成像特性具有极大的潜力，可推动STED显微镜在生物研究中的应用。
Stimulated emission depletion (STED) microscopy can break the optical diffraction barrier and provide subdiffraction resolution. According to the STED superresolution imaging principle, the resolution of STED is positively related to the power of the depletion laser. However, high‐laser power largely limits the study of living cells or living bodies. Moreover, the high complexity and high cost of conventional pulsed STED microscopy limit the application of this technique. Therefore, this paper describes a simple continuous‐wave STED (CW‐STED) system constructed on a 45 × 60 cm breadboard and combined with digitally enhanced (DE) technology; low‐power superresolution imaging is realized, which has the advantages of reducing system complexity and cost. The low‐system complexity, low cost, and low‐power superresolution imaging features of CW‐STED have great potential to advance the application of STED microscopy in biological research.