Experimental Anti-Allergic and Immunomodulatory Effects of Vernonia amygdalina-Derived Biomaterials, Vernodalin and Its Leaf Extracts
Experimental Anti-Allergic and Immunomodulatory Effects of Vernonia amygdalina-Derived Biomaterials, Vernodalin and Its Leaf Extracts
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杏仁斑鸠菊衍生生物材料、斑鸠菊苷及其叶提取物的实验抗过敏和免疫调节作用
DOI：
10.1007/978-981-10-8758-5_10
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Ngatu Nlandu Roger
中科院分区：
文献类型：
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作者：
Hirota Ryoji;Ngatu Nlandu Roger
特应性皮炎（AD）是一种慢性复发性炎症性皮肤病，在发达国家的儿童中发病率相对较高。本研究以NC/Nga小鼠为实验动物，探讨了从苦杏仁斑鸠菊（Vernonia amygdalina，VAM）叶提取物中提取的斑鸠菊素（Vernalin）对NC/Nga小鼠的免疫调节作用，并比较了其抗过敏作用。在第一个实验中，使用2，4，6-三硝基氯苯（TNCB）诱导AD样疾病;在使用SDS溶液破坏皮肤屏障后，将半抗原施加于小鼠嘴侧和耳背区域。AD诱导后，将36只出现中度至重度皮肤病变的NC/Nga小鼠分为6个预处理组，每组6只动物：1 μg/mL维甲酸、10 μg/mL维甲酸、100 μ g/mL维甲酸、1，000 μg/mL VAM提取物、1，000 μg/mL VAM提取物和PBS处理的载体组。处理14天后收集皮肤样品;进行免疫荧光和qRT-PCR测定以分别确定聚丝蛋白（FLG）产生以及FLG mRNA和IL-33 mRNA表达的变化。在第二个实验中，将18只患有屋尘螨提取物（Dermatophagoides pteronyssinusextract，DPE）诱导的AD样疾病的NC/Nga小鼠分为3个治疗组以测试实验药物的疗效：100 μg/mL藜芦醇苷，10 μg/mL VAM浸提液，VAM提取物和藜芦醇苷预处理可引起FLGmRNA表达水平的增加和IL-10表达水平的降低。33 mRNA表达（与对照组相比）。此外，Vernodalin和VAM提取物显着降低了耳部皮肤病变的皮炎评分（与对照组相比）。这项研究的结果证实了VAM衍生的生物活性化合物，藜芦碱和植物提取物的抗过敏作用。
Atopic dermatitis (AD) is a chronic relapsing inflammatory skin disorder with a relatively high prevalence in children in developed countries. In this study, we (1) explored the immunomodulatory and (2) compared the anti-allergic effects ofVernodalinisolated fromVernonia amygdalina(VAM) leaf and VAM leaf extracts in NC/Nga mice. In the first experiment, AD-like disease was induced using 2,4,6-trinitrochlorobenzene(TNCB); the hapten was applied on mice rostral and ear’s dorsal areas after disrupting skin barrier with the use of SDS solution. After AD induction, 36 NC/Nga mice that developed moderate to severe skin lesions were divided into 6 pretreatment groups of 6 animals: 1 μg/mLVernodalin, 10 μg/mLVernodalin, 100 μg/mLVernodalin, 1,000 μg/mL VAM extracts, 1,000 μg/mL VAM extracts, and PBS-treated vehicle group. Skin samples were collected after 14 days of treatment; immunofluorescence and qRT-PCR assays were performed to determine changes in filaggrin (FLG) production and FLGmRNA and IL-33mRNA expressions, respectively. In the second experiment, 18 NC/Nga mice withDermatophagoides pteronyssinusextract (DPE)-induced AD-like disease were divided into 3 treatment groups to test the curative effects of the experimental agents: 100 μg/mLVernodalin, 10 μg/mL VAM extracts, and PBS-treated vehicle group as control.Pretreatment with VAM extracts and Vernodalin caused an increase in FLGmRNA expression levels and a reduction of IL-33mRNA expression in mice (vs. control group). Furthermore,Vernodalinand VAM extracts significantly reduced the dermatitis score (vs. control) in regard to ear skin lesions. Findings from this study confirm the anti-allergic effects of VAM-derived bioactive compound, Vernodalin, and the plant extracts.