Use of the Accessory Genome for Characterization and Typing of Acinetobacter baumannii
Use of the Accessory Genome for Characterization and Typing of Acinetobacter baumannii
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DOI：
10.1128/jcm.02335-10
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发表时间：
2011-04-01
影响因子：
9.4
通讯作者：
Perry, Claire
中科院分区：
文献类型：
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作者：
Turton, Jane F.;Baddal, Buket;Perry, Claire
鲍曼不动杆菌暴发菌株是高度克隆性的，交叉感染调查可能很困难。我们在一组临床分离株(n=70)中，根据Abar耐药岛和在部分(但不是全部)鲍曼不动杆菌中发现的其他基因寻找靶标，这些临床分离株包括许多脉冲场凝胶电泳(PFGE)定义的类型的多个代表。其中包括在两个数量可变的串联重复序列(VNTR)基因座上有不同特征的代表，这可以在PFGE聚类群中提供区分。对所寻求的每个元件的检测或不检测在集合中提供了某种程度的区分，编码噬菌体终止酶(ACICU_02185)、唾液酸合成酶(ACICU_00080)、多糖生物合成蛋白(AB57_0094)、apA1、bla(透射电子显微镜)和整合子相关orfX(京都基因和基因组百科全书)的基因的存在或不存在。K03830)在我们的专家小组中证明了在区分密切相关的菌株方面最有帮助。这些结果支持VNTR数据描述高度相似的不同分离株群体。这种分析与其他分型方法相结合，可以为流行病学调查提供信息，并提供分离株的额外特征。大多数携带BLA(OXA-23-like)的基因型在AbaR4型岛上发现一个yeeA-BLA(OXA-23)片段的聚合酶链式反应阳性，这表明这种现象是普遍存在的。
Outbreak strains of Acinetobacter baumannii are highly clonal, and cross-infection investigations can be difficult. We sought targets based on AbaR resistance islands and on other genes found in some, but not all, sequenced isolates of A. baumannii among a set of clinical isolates (n = 70) that included multiple representatives of a number of pulsed-field gel electrophoresis (PFGE)-defined types. These included representatives that varied in their profiles at two variable-number tandem repeat (VNTR) loci, which can provide discrimination within a PFGE cluster. Detection, or not, of each element sought provided some degree of discrimination among the set, with the presence or absence of genes coding for a phage terminase (ACICU_02185), a sialic acid synthase (ACICU_00080), a polysaccharide biosynthesis protein (AB57_0094), aphA1, bla(TEM), and integron-associated orfX (Kyoto Encyclopedia of Genes and Genomes [KEGG] no. K03830) proving the most helpful in discriminating between closely related isolates in our panel. The results support VNTR data in describing distinct populations of highly similar isolates. Such analysis, in combination with other typing methods, can inform epidemiological investigations and provide additional characterization of isolates. Most genotypes carrying bla(OXA-23-like) were PCR positive for a yeeA-bla(OXA-23) fragment found in an AbaR4-type island, suggesting that this is widespread.