Emergence of nuclear heparanase induces differentiation of human mammary cancer cells
Emergence of nuclear heparanase induces differentiation of human mammary cancer cells
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DOI：
10.1016/j.bbrc.2005.03.129
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发表时间：
2005-05-27
影响因子：
3.1
通讯作者：
Tanaka, N
中科院分区：
文献类型：
--
作者：
Nobuhisa, T;Naomoto, Y;Tanaka, N
上皮细胞分化的研究涉及生物学的许多现代方面。上皮与潜在的间质不断对话，以控制干细胞的活性、转运扩增室的增殖、谱系承诺、终末分化以及最终的细胞死亡。每个活动都有空间上不同的隔间。最近我们报道了肝素酶在细胞核和细胞质中表达，核内肝素酶似乎与细胞分化有关。在这项研究中，我们研究了核内肝素酶在人乳腺上皮癌细胞分化中的作用。我们观察到核肝素酶的表达使细胞分化并出现脂滴。这一发现支持了肝素酶除了其已知的酶功能外在上皮细胞分化中起新作用的观点。(c) 2005爱思唯尔公司版权所有。
The study of epithelial differentiation touches upon many modern aspects of biology. The epithelium is in constant dialogue with the underlying mesenchyme to control stem cell activity, proliferation in transit-amplifying compartments, lineage commitment, terminal differentiation and, ultimately, cell death. There are spatially distinct compartments dedicated to each of these events. Recently we reported that heparanase is expressed in nucleus as well as in the cytoplasm and that nuclear heparanase seems to be related to cell differentiation. In this study, we investigated the role of nuclear heparanase in differentiation by transducing human mammary epithelial cancer cells with heparanase which was delivered specifically into nucleus. We observed that expression of nuclear heparanase allowed the cells to differentiate with the appearance of lipid droplets. This finding supports the idea that heparanase plays a novel role in epithelial cell differentiation apart from its known enzymatic function. (c) 2005 Elsevier Inc. All rights reserved.