Methacrylate monolithic columns of 320 μm ID for capillary liquid chromatography
Methacrylate monolithic columns of 320 μm ID for capillary liquid chromatography
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DOI：
10.1016/s0021-9673(01)01570-9
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发表时间：
2002-02-08
影响因子：
4.1
通讯作者：
Stulík, K
中科院分区：
文献类型：
--
作者：
Coufal, P;Cihák, M;Stulík, K
整体式毛细管柱（320 μ m I.D.）制备的毛细管液相色谱（CLC）的自由基聚合的甲基丙烯酸丁酯（BMA）和乙烯二甲基丙烯酸酯（EDMA）的存在下，致孔剂溶剂包含1-丙醇，丁-1，4-二醇和水。研究了致孔剂溶剂和EDMA含量对整体柱孔隙率和柱效的影响。优化聚合混合物的组成，以获得对于具有各种极性的测试化合物的数十μ m量级的最小HETP。采用货车-Deemter曲线、峰不对称因子、Walters疏水性指数和硅烷醇指数等表征了整体柱的分离性能和选择性。结果表明，320 μ m的I.D.整体柱表现出与100-和150-mum I.D.相似的CLC分离性能。文献中报道的整体柱;此外，320 μ m I.D.色谱柱在CLC条件下操作简单，上样量大。(C)2002 Elsevier Science B. V.保留所有权利。
Monolithic capillary columns (320 mum I.D.) were prepared for capillary liquid chromatography (CLC) by radical polymerization of butylmethacrylate (BMA) and ethylenedimethacrylate (EDMA) in the presence of a porogen solvent containing propan-1-ol, butane-1,4-diol and water. The influence of the contents of the porogen solvent and EDMA in the polymerization mixture on the monolith porosity and column efficiency was investigated. The composition of the polymerization mixture was optimized to attain a minimum HETP of the order of tens of pm for test compounds with various polarities. The separation performance and selectivity of the most efficient monolithic column prepared was characterized by van Deemter curves, peak asymmetry factors and Walters hydrophobicity and silanol indices. It was demonstrated that the 320-mum I.D. monolithic column exhibited CLC separation performance similar to that observed for 100- and 150-mum I.D. monolithic columns reported in the literature; moreover, the 320-mum I.D. column was easier to operate in CLC and exhibited a higher sample loadability. (C) 2002 Elsevier Science B.V. All rights reserved.